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- Clone
- S20006A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Latency Associated Peptide (LAP), Transforming growth factor beta 1 (TGF-b1), TGFB1, DPD1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood mononuclear cells were stimulated with PHA for 2 days. Cells were then stained with GARP (LRRC32) PerCP/Cyanine5.5 and anti-human LAP (TGFβ1 Clone S20006A) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 300007 | 25 tests | 159 CHF | ||||
| 300008 | 100 tests | 375 CHF | ||||
TGF-β1 (transforming growth factor β1) is a multifunctional cytokine which regulates cellular proliferation and differentiation. It is ubiquitously expressed by many types of cells. Platelets express high level of TGF-β. TGF-β is synthesized as a large protein precursor and then secreted as a complex of TGF-β and LAP (latency-associated peptide), in which LAP noncovalently associates with the dimeric mature TGF-β to prevent its activity. TGF-β requires activation before it binds to its receptors and exerts functions. It has been reported that LAP-TGF-β binds to the integrins αvβ1, αvβ6, αvβ8, and α8β1 through RGD domain. TGF-β plays important roles in control proliferation and differentiation of epithelial cells, endothelial cells, fibroblasts, neurons, osteoclasts, and osteoblasts. TGF-β is believed to be important in the regulation of the development of Treg, Th17, and Th9 cells. A recent study has shown that LAP is an activated Treg surface marker.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human TGF-β1 transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone S2006A can completely block the binding of clones TW4-2F8 and TW4-6H10 on target cells.
- RRID
-
AB_2894446 (BioLegend Cat. No. 300007)
AB_2894446 (BioLegend Cat. No. 300008)
Antigen Details
- Structure
- About 400 amino acids, N-terminal signal peptide is required for secretion from a cell, a pro-region (LAP) and C-terminal region that becomes the mature TGF-β molecule following its release from the pro-region by proteolytic cleavage.
- Distribution
-
Ubiquitously expressed by many kinds of cells
- Function
- Multifunctional cytokine, regulates cells proliferation, and differentiation
- Ligand/Receptor
- TGF-βRI, -RII, -RIII, CD105, LTBP, integrins
- Cell Type
- B cells, Dendritic cells, T cells, Tregs
- Biology Area
- Adaptive Immunity, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Inhibitory Molecules, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
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- Yi JJ, et al. 2010. Cell. 142:144.
- Tran DQ, et al. 2009. Blood. 113:5125.
- Lu M, et al. 2002. J Cell Sci. 115:4641.
- Khalil N. 1999. Microbes Infect. 1:1255.
- Gene ID
- 7040 View all products for this Gene ID
- UniProt
- View information about LAP on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All LAP Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human LAP (TGF-β1) | S20006A | FC |
| PE anti-human LAP (TGF-β1) | S20006A | FC |
| APC anti-human LAP (TGF-β1) | S20006A | FC |
| FITC anti-human LAP (TGF-β1) | S20006A | FC |
| PE/Cyanine7 anti-human LAP (TGF-β1) | S20006A | FC |
| TotalSeq™-A1244 anti-human LAP (TGF-β1) | S20006A | PG |
| TotalSeq™-B1244 anti-human LAP (TGF-β1) | S20006A | PG |
| TotalSeq™-C1244 anti-human LAP (TGF-β1) | S20006A | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human LAP (TGF-β1)
Human peripheral blood mononuclear cells were stimulated wit... -
PE anti-human LAP (TGF-β1)
Human peripheral blood mononuclear cells were stimulated wit... -
APC anti-human LAP (TGF-β1)
Human peripheral blood mononuclear cells were stimulated wit... -
FITC anti-human LAP (TGF-β1)
Human peripheral blood mononuclear cells were stimulated wit... -
PE/Cyanine7 anti-human LAP (TGF-β1)
Human peripheral blood mononuclear cells were stimulated wit... -
TotalSeq™-A1244 anti-human LAP (TGF-β1)
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TotalSeq™-B1244 anti-human LAP (TGF-β1)
-
TotalSeq™-C1244 anti-human LAP (TGF-β1)
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