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PE anti-mouse CD62L Antibody

Pricing & Availability
Clone
MEL-14 (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat IgG2a, κ
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Product Citations
publications
MEL-14_PE_090707
C57BL/6 mouse splenocytes stained with MEL-14 PE
  • MEL-14_PE_090707
    C57BL/6 mouse splenocytes stained with MEL-14 PE
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104407 50 µg 110 CHF
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104408 200 µg 293 CHF
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  2. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  3. Chen N, et al. 2021. Front Immunol. 12:756423. PubMed
  4. Pani F, et al. 2021. Endocrinology. 162:. PubMed
  5. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  6. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  7. Song Y, et al. 2020. Front Immunol. 11:558143. PubMed
  8. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  9. Ren S, et al. 2019. Oncol Rep. 41:1509. PubMed
  10. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  11. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  12. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  13. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  14. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  15. Sawada J, et al. 2021. Am J Pathol. 191:396. PubMed
  16. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  17. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  18. Hamasato E, et al. 2014. Brain Behav Immun. 11:109. PubMed
  19. García Nores GD, et al. 2018. Nat Commun. 9:1970. PubMed
  20. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  21. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  22. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  23. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  24. Hiramoto T, et al. 2018. Mol Ther. 26:1255. PubMed
  25. Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
  26. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  27. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  28. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  29. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  30. Ng SS, et al. 2020. Nat Immunol. 21:1205. PubMed
  31. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  32. Green WD, et al. 2022. J Leukoc Biol. 111:147. PubMed
  33. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
  34. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  35. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  36. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  37. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  38. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  39. Zhu X et al. 2019. Cell reports. 27(4):1205-1220 . PubMed
  40. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  41. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  42. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  43. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  44. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  45. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  46. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  47. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  48. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  49. Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed
  50. Li M, et al. 2021. Br J Pharmacol. 178:2617. PubMed
  51. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  52. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  53. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  54. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  55. Rui J, et al. 2021. Nat Commun. 12:5074. PubMed
  56. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  57. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  58. Lee MC, et al. 2017. J Immunol. 198:3565. PubMed
  59. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  60. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  61. Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed
  62. Bantug GR, et al. 2018. Immunity. 48:542. PubMed
  63. Deptuch T, et al. 2022. Sci Rep. 12:13480. PubMed
  64. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  65. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  66. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  67. Cao Y, et al. 2022. Small. 18:e2203466. PubMed
  68. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
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  70. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
RRID
AB_313094 (BioLegend Cat. No. 104407)
AB_313095 (BioLegend Cat. No. 104408)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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