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PE anti-mouse CD54 Antibody

Pricing & Availability
Clone
YN1/1.7.4 (See other available formats)
Regulatory Status
RUO
Other Names
ICAM-1, Ly-47
Isotype
Rat IgG2b, κ
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Product Citations
publications
YN1_1_7_4_PE_083107
C57BL/6 mouse splenocytes stained with YN1/1.7.4 PE
  • YN1_1_7_4_PE_083107
    C57BL/6 mouse splenocytes stained with YN1/1.7.4 PE
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116107 50 µg 110 CHF
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116108 200 µg 317 CHF
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Description

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse NS-1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).

Application References

(PubMed link indicates BioLegend citation)
  1. Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block)
  2. Horley KJ, et al. 1989. EMBO J. 8:2889. (Block)
  3. Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC)
  4. Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP)
  5. Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed
  6. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  7. Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed
  8. Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed
  9. El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed
  10. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Brenner JS, et al. 2018. Nat Commun. 9:20222. PubMed
  2. Cayre S, et al. 2020. Development. 147:00:00. PubMed
  3. Kitur K, et al. 2016. Cell Rep. 16:2219-2230. PubMed
  4. Chao TL, et al. 2020. Front Microbiol. 2.459722222. PubMed
  5. Dúcka M, et al. 2021. Neoplasia. 23:326. PubMed
  6. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  7. Kobori T, et al. 2018. Front Immunol. 9:334. PubMed
  8. Lachenmaier S, et al. 2011. J Neuroimmunol. 232:119. PubMed
  9. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  10. Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed
  11. Bresson L, et al. 2018. Development. 145. PubMed
  12. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  13. Moretti FA, et al. 2018. Elife. 7:e35816. PubMed
  14. Ise W, et al. 2018. Immunity. 48:702. PubMed
  15. Chiche A, et al. 2019. Breast Cancer Res. 21:13. PubMed
  16. Guilliams M, et al. 2022. Cell. 185:379. PubMed
  17. Ponzetta A, et al. 2020. Cell. 178(2):346-360.e24.. PubMed
  18. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
  19. Bordas M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  20. Elgazar-Carmon V, et al. 2008. J Lipid Res. 49:1849. PubMed
  21. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  22. Korin B, et al. 2020. Sleep. :43. PubMed
  23. Corbet M, et al. 2021. PLoS Pathog. 17:e1010069. PubMed
  24. Cayre S, et al. 2020. Development. . PubMed
  25. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  26. Zhong J, et al. 2021. Commun Biol. 4:865. PubMed
RRID
AB_313698 (BioLegend Cat. No. 116107)
AB_313699 (BioLegend Cat. No. 116108)

Antigen Details

Structure
Ig superfamily, 90 kD
Distribution

Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells

Function
Immune reaction, inflammation, adhesion
Ligand/Receptor
CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1990. Nature 346:425.
3. Rothlein R, et al. 1986. J. Immunol. 137:1270.

Gene ID
15894 View all products for this Gene ID
UniProt
View information about CD54 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12.13.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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