- RMP1-14 (See other available formats)
- Regulatory Status
- Other Names
- Programmed Death-1, CD279, PD 1
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
|114117||50 µg||110,00 CHF|
|114118||200 µg||310,00 CHF|
CD279 is a 50-55 kD immunoglobulin superfamily member, also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells).Product Details
- Antibody Type
- Host Species
- Mouse PD-1 transfected BHK cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections1, Western blotting2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC3,4,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114108). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 114110) with a lower endotoxin limit than standard LEAF™ purified antibodies.
(PubMed link indicates BioLegend citation)
- Kanai, T., et al. 2003. J. Immunol. 171:4156. (FC, IHC)
- Personal Communication (WB)
- Yamazaki, T., et al. 2005. J. Immunol. 175:1586. (Costim)
- Matsumoto, K., et al. 2004. J. Immunol. 172:2530. (Block)
- Terrazas, L. I., et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
- Meng, Q., et al. 2006. Invest Opthalmol Vis Sci. 47:444. (FC, WB) PubMed
- Tsang JY, et al. 2011. Int Immunopharmacol. 11:604. PubMed
- Takamura S, et al. 2010. J. Immunol. 184:4696. PubMed (Block)
- Product Citations
AB_2566725 (BioLegend Cat. No. 114117)
AB_2566726 (BioLegend Cat. No. 114118)
- Ig superfamily, 50-55 kD
Subset of double negative thymocytes, activated T and B cells
- Lymphocyte clonal selection, peripheral tolerance
- PD-L1 (B7-H1), PD-L2
- Cell Type
- Thymocytes, T cells, B cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Cancer Biomarkers, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook, Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.
- Gene ID
- 18566 View all products for this Gene ID
- View information about CD279 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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