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PE anti-human IL-17A Antibody

Pricing & Availability
Clone
BL168 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BL168_PE_011409.jpg
PMA (50 ng/ml) + ionomycin (1 µg/ml)-stimulated (6 hours + monensin, 2 µM) human peripheral blood lymphocytes were fixed and permeabilized and then stained with CD3 (UCHT1) APC and anti-human IL-17A (BL168) PE.
  • BL168_PE_011409.jpg
    PMA (50 ng/ml) + ionomycin (1 µg/ml)-stimulated (6 hours + monensin, 2 µM) human peripheral blood lymphocytes were fixed and permeabilized and then stained with CD3 (UCHT1) APC and anti-human IL-17A (BL168) PE.
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512305 25 tests 146 CHF
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512306 100 tests 317 CHF
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Description

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Lu T, et al. 2016. PLoS One. 11: 0148044. PubMed
  2. Tankou SK, et al. 2018. Ann Neurol. 83:1147. PubMed
  3. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  4. Aqel SI, et al. 2021. JCI Insight. 6:e142376. PubMed
  5. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  6. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  7. Ridley ML, et al. 2020. J Immunol. 204:2940. PubMed
  8. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  9. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  10. Wu B, et al. 2020. Cell Metabolism. 32(6):967-980.e5. PubMed
  11. Chruewkamlow N, et al. 2016. PLoS One. 11: 0145983. PubMed
  12. Fraccaroli L, et al. 2015. J Leukoc Biol. 98: 49 - 58. PubMed
  13. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  14. Tomalka J, Jesus T, Ramakrishnan P 2016. J Leukoc Biol. 100: 111 - 120. PubMed
  15. Gabrion A, et al. 2017. J Allergy Clin Immunol. 139:1641. PubMed
  16. Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed
  17. Lichnog C, et al. 2019. Front Pharmacol. 10:39. PubMed
  18. Hr TN, et al. 2021. Allergol Immunopathol (Madr). 49:193. PubMed
  19. Huang LJ, et al. 2021. Immunity. 54(8):1728-1744.e7. PubMed
  20. Lambert EE, et al. 2020. Vaccines (Basel). 8:. PubMed
RRID
AB_961395 (BioLegend Cat. No. 512305)
AB_961394 (BioLegend Cat. No. 512306)

Antigen Details

Structure
Cytokine; dimer; ~15 kD (Mammalian)
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
Activated T cell subpopulation
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Hirota K, et al. 2007. J. Exp. Med. 204:41.
2. Furuzawa-Carballeda J, et al. 2007. Autoimmun. Rev. 6:169.
3. Witowski J, et al. 2007. Kidney Int. 71:514.
4. Gaffen SL, et al. 2006. Vitam. Horm. 74:255.
5. Hymowitz S, et al. 2001. EMBO J. 20:5332.

Gene ID
3605 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02.28.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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