PE anti-human CD24 Antibody

Pricing & Availability
Clone
ML5 (See other available formats)
Regulatory Status
RUO
Workshop
V CD24.5
Other Names
Ly-52, Heat Stable Antigen (HSA), Nectadrin, BA-1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
ML5_PE_062107
Human peripheral blood lymphocytes stained ML5 PE
  • ML5_PE_062107
    Human peripheral blood lymphocytes stained ML5 PE
Compare all formats See PE spectral data
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311105 25 tests 110 CHF
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311106 100 tests 242 CHF
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Description

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York.
  2. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  3. Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF)
  4. Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
Product Citations
  1. Nakazawa Y, et al. 2018. Sci Rep. 8:4013. PubMed
  2. Hao J et al. 2018. Cell metabolism. 28(5):689-705 . PubMed
  3. Lo PK, et al. 2018. Elife. 7:e31334. PubMed
  4. Shah D, et al. 2018. Clin Cancer Res. 24:4566. PubMed
  5. Domínguez–Gómez G, et al. 2018. Mol Med Rep. 17:3397. PubMed
  6. Matossian MD, et al. 2019. BMC Cancer. 19:205. PubMed
  7. Liu T, et al. 2019. Oncol Lett. 18:2262. PubMed
  8. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  9. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  10. Prud'homme G, et al. 2010. PLoS One. 5:e13831. PubMed
  11. Glinka Y, et al. 2012. Biochem Biophys Res Commun. 425:775. PubMed
  12. McClellan S, et al. 2015. Methods. 82: 47-54. PubMed
  13. Vishnoi M, et al. 2015. Sci Rep. 5: 17533. PubMed
  14. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  15. Singh S, et al. 2016. Nature. 538: 109-113. PubMed
  16. Lubaki N, et al. 2016. PLoS Pathog. 12:e1006031. PubMed
  17. Junk D, et al. 2017. Oncogene. 10.1038/onc.2017.33. PubMed
  18. Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed
RRID
AB_314854 (BioLegend Cat. No. 311105)
AB_314855 (BioLegend Cat. No. 311106)

Antigen Details

Structure
GPI-linked glycoprotein, 35-45 kD
Distribution

B cells, granulocytes, epithelial cells

Function
B cell proliferation and differentiation
Ligand/Receptor
CD62P (P-Selectin)
Cell Type
B cells, Epithelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.

Gene ID
100133941 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 08.09.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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