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Pacific Blue™ anti-mouse Lineage Cocktail

Pricing & Availability
Clone
17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status
RUO
Other Names
Lin-1
Isotype
Rat IgG2a/Rat IgG2b
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Product Citations
publications
Lineage_Cocktail_PB_1_112811
C57BL/6 bone marrow cells were stained with CD117 FITC and Pacific Blue™ lineage cocktail (top plot) or Pacific Blue™ isotype control cocktail (bottom plot).
  • Lineage_Cocktail_PB_1_112811
    C57BL/6 bone marrow cells were stained with CD117 FITC and Pacific Blue™ lineage cocktail (top plot) or Pacific Blue™ isotype control cocktail (bottom plot).
  • Lineage_Cocktail_PB_2_112811
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133310 100 tests 546 CHF
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Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Pacific Blue™ Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119.

 

Materials provided:
1. Pacific Blue™ anti-mCD3/ Pacific Blue™ anti-mLy-6G(Ly-6C)/ Pacific Blue™ anti-mCD11b/ Pacific Blue™ anti-mCD45R(B220)/ Pacific Blue™ anti-mTer-119

Materials not provided:
1. Cell Staining Buffer (Cat. No. 420201)
2. Isotype Controls

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Host Species
Rat
Formulation
This set contains Pacific Blue™ conjugated antibodies in an optimal concentration for flow cytometric analysis.
Preparation
The antibodies are purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. He Y, et al. 2021. Infect Immun. 89:. PubMed
  2. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  3. Labuhn M, et al. 2020. Cancer Cell. 36(2):123-138. PubMed
  4. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  5. Dráberová L, et al. 2020. Front Immunol. 10:3068. PubMed
  6. Simon M, et al. 2019. Cell Metab. 29:871. PubMed
  7. Lukowski SW et al. 2019. Cell Rep. 27(9):2748-2758 . PubMed
  8. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  9. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  10. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  11. Frech S, et al. 2021. J Invest Dermatol. . PubMed
  12. Mistry JJ, et al. 2021. Nat Commun. 12:7130. PubMed
  13. Capitano ML, et al. 2019. J Clin Invest. 130:2555. PubMed
  14. Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed
  15. van Gastel N, et al. 2020. Cell Metabolism. 32(3):391-403.e6. PubMed
  16. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  17. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  18. Capitano ML, et al. 2021. J Clin Invest. 131:. PubMed
  19. Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed
  20. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID
AB_11150779 (BioLegend Cat. No. 133310)

Antigen Details

Distribution

T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes

Cell Type
B cells, Erythrocytes, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area
Immunology
Antigen References

1. Morrison SJ, et al. 1997. Development 124:1929.
2. Okada S, et al. 1992. Blood 80:3044.
3. Spangrude GJ, et al. 1988. Science 241:58.
4. Spangrude GJ, et al. 1990. Exp. Hematol. 18:920.
5. Tan SL, et al. 2006. J. Immunol. 176:2872.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about Lineage on UniProt.org

Other Formats

View All Lineage Reagents Request Custom Conjugation
Description Clone Applications
Pacific Blue™ anti-mouse Lineage Cocktail with Isotype Ctrl 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Pacific Blue™ anti-mouse Lineage Cocktail 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Brilliant Violet 421™ anti-mouse Lineage Cocktail 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Alexa Fluor® 700 anti-mouse Lineage Cocktail with Isotype Ctrl 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Go To Top Version: 3    Revision Date: 07.09.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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