LEAF™ Purified anti-human EGF Antibody

Pricing & Availability
Clone
Poly5235 (See other available formats)
Regulatory Status
RUO
Other Names
Urogastrone (URG), HOMG4
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
1_Poly5235_LEAF_EGF_Antibody_110821
Recombinant human EGF (Cat. No. 585506) (black circles) inhibits proliferation on human epithelial A431 cells. LEAF™ purified anti-human EGF antibody (Poly5235) (purple squares) neutralizes the cell proliferation inhibited by recombinant human EGF at 4 ng/mL in human epithelial A431 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG, isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 0.1 - 0.5 μg/mL.
  • 1_Poly5235_LEAF_EGF_Antibody_110821
    Recombinant human EGF (Cat. No. 585506) (black circles) inhibits proliferation on human epithelial A431 cells. LEAF™ purified anti-human EGF antibody (Poly5235) (purple squares) neutralizes the cell proliferation inhibited by recombinant human EGF at 4 ng/mL in human epithelial A431 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG, isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 0.1 - 0.5 μg/mL.
  • 2_Poly5235_LEAF_EGF_Antibody_072722
    Whole cell extracts (15 µg total protein) from HeLa, and NIH/3T3 cells spiked with or without the indicated amount of Recombinant human EGF protein (Cat. No. 585506) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL purified anti-human EGF (clone Poly5235) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-goat. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker
  • 3_Poly5235_LEAF_EGF_Antibody_072722
    IHC staining of anti-human EGF (clone Poly5235) on formalin-fixed paraffin-embedded human kidney (A) and human spleen (B). Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with (+) or without (-) 10.0 μg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm
Cat # Size Price Quantity Check Availability Save
523503 100 µg 413 CHF
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Description

Epidermal growth factor (EGF) is a small 6 kD polypeptide and has six conserved cysteine residues that form three intramolecular disulfide bonds. Human and mouse EGF share 70% homology in amino acid structure. Human EGF is synthesized as a transmembrane precursor protein (1207 amino acids) which is proteolytically cleaved to generate the 54 amino acid mature EGF. Many different cells including mammary gland cells, macrophages, gut epithelial cells, and cells in the nervous system and the kidney can produce EGF. EGF plays important roles in the regulation of cell survival, proliferation, and differentiation by binding to its receptor EGFR. For example, EGF can stimulate the proliferation of mouse embryonic stem cells or induce the terminal differentiation/growth inhibition of A431 cells. The binding of EGF to EGFR will induce receptor dimerization, which is required for activating the tyrosine kinase in the receptor cytoplasmic domain. In addition, the binding of EGF to its receptor triggers several signal transduction pathways including JAK/STAT, Ras/ERK, and PI3K/AKT pathways. Blocking of the EGF/EGFR pathway can suppress some tumor cell's proliferation. Other members of the EGF family (including transforming growth factor-α (TGF-α), heparin-binding EGF-like growth factor (HB-EGF), amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), and epigen also bind to EGFR.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human EGF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
WB, IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the inhibited proliferation induced by recombinant human EGF (Cat. No. 585506) at 4 ng/mL on human epithelial A431 cells. ND50 range: 0.1 - 0.5 µg/mL. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2904433 (BioLegend Cat. No. 523503)

Antigen Details

Structure
Growth factor
Distribution

Mammary gland cell, macrophage, gut epithelial cells, cells in the nervous system, kidney

Function
EGF is a potent mitogen for many cells in culture, and in vivo, it induces the proliferation and differentiation of skin, cornea, lung, and trachea, among other tissues. Processing of pro EGF to mature EGF in different tissues is not equally efficient. The precursor is processed to mature EGF in the submaxillary gland, pancreas, small intestine, and mammary gland. In the submaxillary gland, EGF is fully processed, stored at secretory granules, and secreted in saliva. In kidney, EGF is present in unprocessed or intermediated forms on the cell surface.
Interaction
Epithelial cells, epidermal cells, and fibroblasts
Ligand/Receptor
EGFR
Antigen References
  1. Henson ES and Gibson SB. 2006. Cell Signal. 18:2089.
  2. Burgess AW, et al. 2003. Mol. Cell. 12:541.
  3. Imai Y, et al. 1982. Cancer Res. 42:4394.
  4. Barnes DW. 1982. J. Cell. Biol. 93:1.
  5. Heo JS, et al. 2006. Am. J. Physiol. Cell. Physiol. 290:C123.
Gene ID
1950 View all products for this Gene ID
UniProt
View information about EGF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 07.27.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • LEAF™ Purified anti-human EGF

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