Human peripheral blood lymphocytes and monocytes were stained with True-Stain Monocyte Blocker™ (Cat. No. 426103), CD123 FITC, and CD303 (BDCA-2, clone 201A) APC/Fire™ 750 (left) or mouse IgG2a, κ APC/Fire™ 750 isotype control (right).
Human peripheral blood lymphocytes and monocytes were stained with True-Stain Monocyte Blocker™ (Cat. No. 426103), CD123 FITC, and CD303 (BDCA-2, clone 201A) APC/Fire™ 750 (left) or mouse IgG2a, κ APC/Fire™ 750 isotype control (right).
CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.
Excitation Laser
Red Laser (633 nm)
Application References
(PubMed link indicates BioLegend citation)
Freeman A, et al. 2014. PLoS One. 9:110928. PubMed
Johnson P, et al. 2015. Clin Cnacer Res. 21:1321. PubMed
1. Jähn PS, et al. 2010. Cell Immunol. 265:15.
2. Graham LM and Brown GD. 2009. Cytokine 48:148.
3. Röck J, et al. 2007. Eur. J. Immunol. 37:3564.
4. Dzionek A, et al. 2001. J. Exp. Med. 194:1823.
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