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APC/Cyanine7 anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119_APCCyanine7_TER119-Erythroid_Antibody_020119
C57BL/6 bone marrow cells were stained with TER-119 APC/Cyanine7 (filled histogram) or Rat IgG2b, ? APC/Cyanine7 isotype control (open histogram). Data shown was gated on small scatter population.
  • TER-119_APCCyanine7_TER119-Erythroid_Antibody_020119
    C57BL/6 bone marrow cells were stained with TER-119 APC/Cyanine7 (filled histogram) or Rat IgG2b, ? APC/Cyanine7 isotype control (open histogram). Data shown was gated on small scatter population.
Compare all formats See APC/Cyanine7 spectral data
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116223 25 µg 94 CHF
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  2. Casellas Roman HL, et al. 2020. Leuk Res. 94:106372. PubMed
  3. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  4. Gawish R, et al. 2022. Elife. 11:. PubMed
  5. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  6. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  7. Cheung P, et al. 2020. Cell Stem Cell. 27(4):590-604.e9. PubMed
  8. Chen S, et al. 2022. Cancer Discov. :. PubMed
  9. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  10. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  11. Castiglioni A, et al. 2015. PLoS One. 10:128094. PubMed
  12. Lu SX, et al. 2021. Cell. . PubMed
  13. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  14. Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed
  15. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  16. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  17. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  18. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  19. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  20. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  21. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  22. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  23. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  24. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  25. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  26. Xu R, et al. 2018. Nat Med. 24:823. PubMed
  27. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  28. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  29. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  30. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  31. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  32. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  33. Ji G, et al. 2019. Bone Joint J. 101-B:108. PubMed
  34. Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed
  35. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  36. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  37. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  38. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  39. Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
  40. Kalamakis G, et al. 2019. Cell. 176:1407. PubMed
  41. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  42. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  43. Venugopal J, et al. 2020. Sci Rep. 10:16514. PubMed
  44. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  45. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  46. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  47. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  48. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  49. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  50. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  51. Beiter RM, et al. 2022. Sci Rep. 12:12921. PubMed
  52. McKenney AS, et al. 2018. J Clin Invest. 128:789. PubMed
  53. Wang L, et al. 2020. Nat Commun. 0.654166667. PubMed
  54. Xu X, et al. 2011. Am J Physiol Regul Integr Comp Physiol. 300:R1115. PubMed
  55. Fast EM, et al. 2021. Elife. 10:. PubMed
  56. Angueira AR, et al. 2021. Nat Metab. 3:469. PubMed
  57. Gawish R, et al. 2022. Elife. 11:. PubMed
  58. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
  59. Rossi G, et al. 2022. Sci Rep. 12:13380. PubMed
  60. Li Z, et al. 2021. J Mol Histol. . PubMed
RRID
AB_2137788 (BioLegend Cat. No. 116223)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Other Formats

View All TER-119 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TER-119/Erythroid Cells TER-119 FC
Biotin anti-mouse TER-119/Erythroid Cells TER-119 FC
FITC anti-mouse TER-119/Erythroid Cells TER-119 FC
PE anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Pacific Blue™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready) TER-119 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB,CMCD
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody TER-119 FC
Brilliant Violet 711™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Go To Top Version: 3    Revision Date: 02.01.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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