Ultra-LEAF™ Purified anti-CXCL12 (SDF-1β) Antibody

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Recombinant mouse CXCL12 (SDF-1β)

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CXCL12 (SDF-1β) (0.25 ng/mL) on mouse Baf3-hCXCR4 transfectant chemoattractant cells. ND₅₀ range: 0.05 - 0.3 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CXCL12 (SDF-1α) (1.5 ng/mL) on mouse Baf3-hCXCR4 transfectant chemoattractant cells. ND₅₀ range: 0.05 - 0.3 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CXCL12 (SDF-1α) (0.15 ng/mL) on mouse Baf3-hCXCR4 transfectant chemoattractant cells. ND₅₀ range: 0.02 - 0.12 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CXCL12 (SDF-1β) (0.12 μg /mL) on mouse Baf3-hCXCR4 transfectant chemoattractant cells. ND₅₀ range: 0.8 - 4 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID

AB_2894525 (BioLegend Cat. No. 939903)
AB_2894525 (BioLegend Cat. No. 939904)

Antigen Details

Structure

Chemokine

Distribution

Expressed by bone marrow stromal cells, pericytes, fibroblasts, endothelial cells, epithelial cells, dendritic cells, astrocytes, hematopoietic stem and progenitors, leukocytes, lymphocytes, macrophages, megakaryocytes, microglia, monocytes, neuron stem cells, neurons

Function

CXCL12 is a constitutive chemokine involved in lung, brain, joint, and intestine inflammation. CXCL12 is chemotactic for T, B, and cancer cells. CXCL12/CXCR4 pair plays a role in mobilization of CD34⁺ progenitors to peripheral blood. CXCL12 is important in morphogenesis, angiogenesis, cancer metastasis, neurogenesis and neuroinflammation.

Interaction

Endothelial cells, epithelial cells, T and B cells, monocytes

Ligand/Receptor

CXCR4

Cell Type

Astrocytes, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Leukocytes, Lymphocytes, Macrophages, Megakaryocytes, Microglia, Monocytes, Neural Stem Cells, Neurons

Biology Area

Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Death, Immuno-Oncology, Immunology, Innate Immunity, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance, Stem Cells

Molecular Family

Adhesion Molecules, Cytokines/Chemokines, GPCR

Antigen References

1. Nagasawa T, et al. 1994. Proc Natl Acad Sci USA. 91:2305.
2. Muller A, et al. 2001. Nature. 410:50.
3. Valenzuela-Fernandez A, et al. 2002. J Biol Chem. 277:15677.
4. De La Luz Sierra M, et al. 2004. Blood. 103:2452.
5. Kortesidis A, et al. 2005. Blood. 105:3793.
6. Davis DA, et al. 2005. Blood. 105:4561.
7. Zlotnik A. 2008. J Pathol. 215:211.
8. Herberg S, et al. 2013. PLoS One. 8:e58207.

Gene ID

6387 View all products for this Gene ID

UniProt

View information about CXCL12 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis