- MOPC-21 (See other available formats)
- Regulatory Status
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.Product Details
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The immunoglobulin was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The IgG1, κ isotype control solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC, ICFC - Quality tested
- Recommended Usage
Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed
- Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA)
- Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed
- Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF)
- Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
- Lian IA, et al. 2011. Placenta. 32:823. PubMed
- Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
- Product Citations
AB_893680 (BioLegend Cat. No. 400149)
AB_893664 (BioLegend Cat. No. 400150)
- Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- IgG1 kappa
- Specificity Alt (DOES NOT SHOW ON TDS):
- IgG1, κ
- View information about IgG1 kappa on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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