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PerCP/Cyanine5.5 anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5-114-15-2_PerCPCyanine55_I-A-I-E_Antibody_101618
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC and I-A/I-E (clone M5/114.15.2) PerCP/Cyanine5.5 (left), or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
  • M5-114-15-2_PerCPCyanine55_I-A-I-E_Antibody_101618
    C57BL/6 mouse splenocytes were stained with CD45R/B220 APC and I-A/I-E (clone M5/114.15.2) PerCP/Cyanine5.5 (left), or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
107625 25 µg $118
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107626 100 µg $323
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  2. Ortiz G, et al. 2020. Front Immunol. 11:1713. PubMed
  3. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
  4. Gonçalves NP, et al. 2017. J Neuroinflammation. 14:115. PubMed
  5. Matsumoto R, et al. 2021. Front Pharmacol. 12:715752. PubMed
  6. Kong D, et al. 2022. J Heart Lung Transplant. Online ahead of print. PubMed
  7. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  8. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  9. Gounder AP, et al. 2018. PLoS Pathog. 14:e1007001. PubMed
  10. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  11. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  12. Li S, et al. 2021. Ann Transl Med. 9:1644. PubMed
  13. Volkov A, et al. 2013. PLoS One. 8:60565. PubMed
  14. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  15. Sharma PK, et al. 2018. Cancer Gene Ther. 25:27. PubMed
  16. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  17. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  18. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  19. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  20. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  21. Pello O, et al. 2012. PLoS One. 7:e45399. PubMed
  22. Vijay R, et al. 2015. J Exp Med. 212: 1851 - 1868. PubMed
  23. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  24. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  25. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  26. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  27. McFarlane AJ, et al. 2017. J Allergy Clin Immunol. 140:1068. PubMed
  28. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  29. Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed
  30. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  31. Xue N, et al. 2017. Sci Rep. 7:39011. PubMed
  32. Ji DX, et al. 2019. Nat Microbiol. 1.644444444. PubMed
  33. Yang Y, et al. 2021. Sci Adv. 7:eabf6290. PubMed
  34. Zhang Q, et al. 2022. Nat Commun. 13:2406. PubMed
  35. Nishida J, et al. 2022. STAR Protoc. 3:101306. PubMed
  36. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  37. Bartmann J, et al. 2016. Int Immunol. 28: 473 - 487. PubMed
  38. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  39. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  40. Movahedi K, et al. 2012. Cancer Res. 74:4165. PubMed
  41. Fu Y, et al. 2021. Front Cell Dev Biol. 9:689727. PubMed
  42. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  43. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  44. Yi H, et al. 2021. Front Immunol. 12:719189. PubMed
  45. Clement CC, et al. 2021. Immunity. 54:721. PubMed
  46. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  47. Lu X, et al. 2019. Circ Res. 125:1055. PubMed
  48. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  49. Walker MT, et al. 2021. Front. Allergy. 2:. PubMed
  50. Bell CR, et al. 2022. Nat Commun. 13:2063. PubMed
  51. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  52. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  53. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  54. Aguilar-Pimentel A, et al. 2017. PLoS One. 12(6):e0178563. PubMed
  55. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  56. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  57. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  58. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  59. Golabek A, et al. 2021. Exp Ther Med. 21:482. PubMed
  60. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  61. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  62. Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
  63. Shi GN, et al. 2021. Ther Adv Med Oncol. 13:1758835920987056. PubMed
  64. Li J, et al. 2021. eLife. 10:00. PubMed
  65. Chng S, et al. 2016. Sci Rep. 6: 23820. PubMed
RRID
AB_2191072 (BioLegend Cat. No. 107625)
AB_2191071 (BioLegend Cat. No. 107626)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10/30/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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