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PerCP/Cyanine5.5 anti-mouse CD48 Antibody

Pricing & Availability
Clone
HM48-1 (See other available formats)
Regulatory Status
RUO
Other Names
BCM1, SLAMF2, Blast-1
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
HM48-1_PerCPCyanine55_CD48_Antibody_022219
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48-1) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
  • HM48-1_PerCPCyanine55_CD48_Antibody_022219
    C57BL/6 mouse splenocytes were stained with CD48 (clone HM48-1) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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103421 25 µg $141
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103422 100 µg $347
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Description

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
Product Citations
  1. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  2. Chen S, et al. 2022. Cancer Discov. :. PubMed
  3. Krzeptowski W, et al. 2021. Antioxidants (Basel). 10:00. PubMed
  4. Szade K, et al. 2013. PLoS One. 8:63329. PubMed
  5. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  6. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  7. Arai F, et al. 2020. Cell Systems. 11(6):640-652.e5. PubMed
  8. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  9. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  10. Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed
  11. Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed
  12. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  13. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
  14. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
RRID
AB_1575045 (BioLegend Cat. No. 103421)
AB_2075051 (BioLegend Cat. No. 103422)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 02/22/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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