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PerCP/Cyanine5.5 anti-mouse CD107a (LAMP-1) Antibody

Pricing & Availability
Clone
1D4B (See other available formats)
Regulatory Status
RUO
Other Names
Lysosome-associated membrane glycoprotein 1, lysosomal membrane glycoprotein 1, CD107 antigen-like family member A, lysosome-associated membrane protein 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1D4B_PerCPCy5.5_CD107a_(LAMP-1)_Antibody_FC_032516
Thioglycollate-elicited Balb/c mouse peritoneal macrophages were stained with CD107a (clone 1D4B) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 1D4B_PerCPCy5.5_CD107a_(LAMP-1)_Antibody_FC_032516
    Thioglycollate-elicited Balb/c mouse peritoneal macrophages were stained with CD107a (clone 1D4B) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
121625 25 µg $112
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121626 100 µg $276
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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Product Citations
  1. Park S, et al. 2021. Front Immunol. 11:620166. PubMed
  2. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  3. Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed
  4. van Vloten JP, et al. 2019. Mol Ther Methods Clin Dev. 13:154. PubMed
  5. Lind L, et al. 2021. J Neurovirol. 27:145. PubMed
  6. López-Sanz C, et al. 2022. STAR Protoc. 3:101755. PubMed
  7. Li Y, et al. 2022. Theranostics. 12:5364. PubMed
  8. Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed
  9. Zhang X, et al. 2019. Front Immunol. 10:744. PubMed
RRID
AB_2572054 (BioLegend Cat. No. 121625)
AB_2572055 (BioLegend Cat. No. 121626)

Antigen Details

Structure
LAMP-1 is a 406 amino acid protein with a molecular mass of 44 kD.
Gene ID
16783 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 03/25/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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