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PE/Cyanine5 anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
M1slash70_PECy5_042706
C57BL/6 mouse bone marrow cells stained with CD11b (clone M1/70) PE/Cyanine5 (filled histogram) or rat IgG2b, κ PE/Cyanine5 isotype control (open histogram) (gated on total cells).
  • M1slash70_PECy5_042706
    C57BL/6 mouse bone marrow cells stained with CD11b (clone M1/70) PE/Cyanine5 (filled histogram) or rat IgG2b, κ PE/Cyanine5 isotype control (open histogram) (gated on total cells).
Compare all formats See PE/Cyanine5 spectral data
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101209 25 µg $77
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101210 100 µg $220
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Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee, Baboon, Rabbit
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  35. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  36. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Ponomarev E, et al. 2006. J Immunol. 176:1402. PubMed
  2. Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed
  3. Kurosawa M, et al. 2021. Int J Mol Sci. 22:. PubMed
  4. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  5. Søndergaard E, et al. 2020. Cell Reports. 29(9):2756-2769.e6.. PubMed
  6. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  7. Kinder JM, et al. 2020. Cell Reports. 31(12):107784.. PubMed
  8. Wei H, et al. 2020. Cell Death Dis. 11:290. PubMed
  9. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  10. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  11. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  12. Godbersen-Palmer C, et al. 2020. J Immunol. 204:2973. PubMed
  13. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  14. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
  15. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  16. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  17. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  18. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  19. Heger L, et al. 2018. Front Immunol. 9:744. PubMed
  20. Cervera–Carrascon V, et al. 2020. Oncoimmunology. 9:1761229. PubMed
  21. Amouzegar A, et al. 2017. Stem Cells. 10.1002/stem.2611. PubMed
  22. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  23. Yankova E, et al. 2021. Nature. 593:597. PubMed
  24. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  25. Yang C, et al. 2021. J Invest Dermatol. 810:141. PubMed
  26. Dawar S, et al. 2016. Cell Death Dis. 7:e2509. PubMed
  27. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  28. Wara AK, et al. 2020. Cell Rep. 33:108550. PubMed
  29. Ghandhi SA, et al. 2019. Sci Rep. 9:19434. PubMed
  30. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  31. Wang SK, et al. 2021. JCI Insight. 6: . PubMed
  32. Severance AL, et al. 2022. iScience. 25:104400. PubMed
  33. Zervantonakis I, et al. 2012. Proc Natl Acad Sci U S A. 109:13515. PubMed
  34. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  35. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  36. Hsu M, et al. 2022. Nat Immunol. 23:581. PubMed
  37. Wilkes MC, et al. 2020. Nat Commun. 2.780555556. PubMed
  38. Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed
  39. Kaiser T, et al. 2019. Eneuro. 6. PubMed
  40. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  41. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  42. Barbieri I, et al. 2017. Nature. 552:126. PubMed
  43. Azzoni E, et al. 2021. Cell Rep. 37:110103. PubMed
  44. Koide S, et al. 2022. iScience. 25:103603. PubMed
  45. Wilkes MC, et al. 2021. J Biol Chem. 297:100988. PubMed
  46. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  47. Béchade C, et al. 2020. Glia. . PubMed
  48. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  49. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  50. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  51. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  52. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  53. Chen D, et al. 2021. JCI Insight. 6:. PubMed
  54. Rowe R, et al. 2016. J Exp Med. 213: 1497 - 1512. PubMed
  55. Shao TY et al. 2019. Cell host & microbe. 25(3):404-417 . PubMed
  56. Morales J, et al. 2014. J Leukoc Biol. 95:643. PubMed
RRID
AB_312792 (BioLegend Cat. No. 101209)
AB_312793 (BioLegend Cat. No. 101210)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD11b Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD11b M1/70 FC
Biotin anti-mouse/human CD11b M1/70 FC
FITC anti-mouse/human CD11b M1/70 FC
PE anti-mouse/human CD11b M1/70 FC,IHC
PE/Cyanine5 anti-mouse/human CD11b M1/70 FC
Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,IHC-F,ICC
PE/Cyanine7 anti-mouse/human CD11b M1/70 FC
Alexa Fluor® 488 anti-mouse/human CD11b M1/70 FC,IHC-F,3D IHC,SB
Alexa Fluor® 647 anti-mouse/human CD11b M1/70 FC,3D IHC,SB
Alexa Fluor® 700 anti-mouse/human CD11b M1/70 FC
Pacific Blue™ anti-mouse/human CD11b M1/70 FC
APC/Cyanine7 anti-mouse/human CD11b M1/70 FC
PerCP/Cyanine5.5 anti-mouse/human CD11b M1/70 FC
PerCP anti-mouse/human CD11b M1/70 FC
Brilliant Violet 421™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 570™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 605™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 650™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 711™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 785™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 510™ anti-mouse/human CD11b M1/70 FC,ICC
Ultra-LEAF™ Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,Block,Depletion,IHC-F,ICC
Purified anti-mouse/human CD11b (Maxpar® Ready) M1/70 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD11b M1/70 IHC-F,FC
PE/Dazzle™ 594 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 750 anti-mouse/human CD11b M1/70 FC
TotalSeq™-A0014 anti-mouse/human CD11b M1/70 PG
Brilliant Violet 750™ anti-mouse/human CD11b M1/70 FC
TotalSeq™-B0014 anti-mouse/human CD11b M1/70 PG
TotalSeq™-C0014 anti-mouse/human CD11b M1/70 PG
Spark NIR™ 685 anti-mouse/human CD11b M1/70 FC
PE/Fire™ 640 anti-mouse/human CD11b M1/70 FC
Spark YG™ 593 anti-mouse/human CD11b M1/70 FC
Spark YG™ 570 anti-mouse/human CD11b M1/70 IHC-F
PE/Fire™ 810 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 810 anti-mouse/human CD11b Antibody M1/70 FC
Spark Blue™ 550 anti-mouse/human CD11b M1/70 FC
Spark UV™ 387 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 806 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 780 anti-mouse/human CD11b M1/70 FC
Spark Blue™ 574 anti-mouse/human CD11b (Flexi-Fluor™) M1/70 FC
Spark PLUS UV™ 395 anti-mouse/human CD11b M1/70 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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