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FITC anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
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Product Citations
publications
145-2C11_FITC_021606
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) FITC and CD127 (clone SB/199) PE.
  • 145-2C11_FITC_021606
    C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) FITC and CD127 (clone SB/199) PE.
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100305 50 µg $30
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100306 500 µg $99
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Chei S, et al. 2020. Front Nutr. 7:583186. PubMed
  2. Bertrand F, et al. 2017. Nat Commun. 8:2256. PubMed
  3. Katsumura KR, et al. 2018. Proc Natl Acad Sci U S A. 115:E10109. PubMed
  4. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  5. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  6. Zhou J, et al. 2021. Int J Med Sci. 18:3516. PubMed
  7. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  8. Schulteis R, et al. 2008. Blood. 112:4905. PubMed
  9. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  10. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  11. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  12. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  13. Fornari T, et al. 2015. PLoS One. 10: 0142688. PubMed
  14. Koning B, et al. 2006. Int Immunol. 1.403472222. PubMed
  15. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  16. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  17. Tian H, et al. 2022. Cancer Sci. 113:875. PubMed
  18. Lou F, et al. 2020. STAR Protoc. 1:100115. PubMed
  19. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  20. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  21. Allegrezza M, et al. 2016. Cancer Res . 76: 6253 - 6265. PubMed
  22. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  23. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  24. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  25. Patnode M, et al. 2013. Glycobiology. 23:381. PubMed
  26. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  27. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  28. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  29. Uchida A, et al. 2021. J Inflamm Res. 14:3089. PubMed
  30. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  31. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  32. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  33. Peng L, et al. 2020. Transl Lung Cancer Res. 0.892361111. PubMed
  34. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  35. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  36. Li H, et al. 2022. Theranostics. 12:6422. PubMed
  37. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  38. Yin Q, et al. 2015. PLoS One. 10: 0137808. PubMed
  39. Ma Y, et al. 2015. J Immunol. 195: 3769 - 3780. PubMed
  40. Matsumura K, et al. 2016. J Immunol. 197: 3233 - 3244. PubMed
  41. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  42. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  43. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  44. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  45. Jeong SH, et al. 2021. Nat Commun. 12:4405. PubMed
  46. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  47. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  48. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  49. Skeate JG, et al. 2018. PLoS One. 13:e0191311. PubMed
  50. He L, et al. 2019. iScience. 0.790972222. PubMed
  51. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  52. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  53. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  54. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  55. Yang H, et al. 2022. Nutrients. 14:. PubMed
  56. Kondratieva T, et al. 2020. PLoS One. 15:e0239668. PubMed
  57. Miki Y, et al. 2016. J Biol Chem. 291: 15588 - 15601. PubMed
  58. Tang CC, et al. 2021. eLife. 10:00. PubMed
  59. Gong Y, et al. 2020. Cell Metabolism. 33(1):51-64.e9. PubMed
  60. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  61. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  62. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  63. Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed
  64. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  65. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  66. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  67. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  68. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  69. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  70. Yi X, et al. 2020. Sci Adv. 6:eaba3546. PubMed
  71. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  72. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  73. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  74. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  75. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  76. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  77. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  78. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  79. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  80. Bian G, et al. 2020. British Journal of Pharmacology. 178(3):636-653. PubMed
  81. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  82. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  83. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  84. Kim MY, et al. 2017. Plant Biotechnol J. 1.729166667. PubMed
  85. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  86. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  87. Xu M, et al. 2013. J Immunol. 190:5436. PubMed
  88. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  89. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  90. Labiano S, et al. 2017. Oncoimmunology. 6:e1283468. PubMed
  91. Engblom C, et al. 2017. Science. 358:6367. PubMed
  92. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  93. Lind L, et al. 2021. J Neurovirol. 27:145. PubMed
  94. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  95. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  96. Soria-Castro R, et al. 2020. Sci Rep. 10:17802. PubMed
  97. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  98. Eisemann T, et al. 2019. Front Oncol. 9:187. PubMed
  99. Yue W, et al. 2019. Nat Commun. 10:2025. PubMed
  100. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  101. Sánchez-del-Campo L, et al. 2021. J Exp Clin Cancer Res. 40:117. PubMed
  102. Casselli T, et al. 2021. PLoS Pathog. 17:e1009256. PubMed
  103. Okada R, et al. 2021. EBioMedicine. 67:103345. PubMed
  104. Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed
  105. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  106. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  107. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
  108. Lin JC, et al. 2019. Nat Commun. 10:1057. PubMed
  109. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  110. La Salvia S, et al. 2020. Am J Physiol Renal Physiol. 319:F868. PubMed
  111. Fitzpatrick Z, et al. 2020. Nature. 587:472. PubMed
  112. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  113. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  114. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  115. Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
  116. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  117. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  118. Ma C, et al. 2021. Mol Metab. 47:101170. PubMed
  119. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  120. Sheng J, et al. 2021. eLife. 10:00. PubMed
  121. Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed
  122. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  123. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  124. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  125. Schofield L, et al. 2017. BMC Med. 10.1186/s12916-017-0883-8. PubMed
  126. Zhou X, et al. 2021. Nutr Metab (Lond). 18:64. PubMed
  127. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  128. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  129. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
  130. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
  131. L M, et al. 2016. Brain. 139: 1939-1957. PubMed
  132. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  133. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  134. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  135. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  136. Kanaya T, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 295:273. PubMed
  137. Amatruda M, et al. 2022. Brain Commun. 4:fcac044. PubMed
  138. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_312670 (BioLegend Cat. No. 100305)
AB_312671 (BioLegend Cat. No. 100306)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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