Brilliant Violet 421™ anti-mouse VISTA (PD-1H) Antibody

Pricing & Availability
MIH63 (See other available formats)
Regulatory Status
Other Names
PD-1 homolog, Dies1
Rat IgG2a, κ
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Product Citations
C57BL/6 mouse splenocytes were stained with CD8 APC/Fire™ 750 and PD-1H (clone MIH63) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
  • MIH63_BV421_VISTA_Antibody_032718
    C57BL/6 mouse splenocytes were stained with CD8 APC/Fire™ 750 and PD-1H (clone MIH63) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
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150212 50 µg $292
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PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein that is composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and natural killer cells (NK). It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants due to its ability to preferentially suppress CD4+ T cell-mediated immunity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse VISTA transfected J558 cells.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
AB_2728192 (BioLegend Cat. No. 150212)

Antigen Details

Type I transmembrane protein, member of the CD28 family.

Macrophages, dendritic cells, neutrophils, natural killer cells, and naive and activated T cells.

Negative regulator of the immune response.
Cell Type
Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Antigen References

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp. Med. 208:577.
3. Flies DB, et al. 2014. J. Clin. Invest. 124:1966.

Gene ID
74048 View all products for this Gene ID
View information about VISTA on

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 03/28/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse VISTA (PD-1H)

  • PE anti-mouse VISTA (PD-1H)

  • APC anti-mouse VISTA (PD-1H)

  • Biotin anti-mouse VISTA (PD-1H)

  • Brilliant Violet 421™ anti-mouse VISTA (PD-1H)

  • PerCP/Cyanine5.5 anti-mouse VISTA (PD-1H)

  • PE/Dazzle™ 594 anti-mouse VISTA (PD-1H)

  • PE/Cyanine7 anti-mouse VISTA (PD-1H)

  • TotalSeq™-A0915 anti-mouse VISTA (PD-1H)

  • TotalSeq™-C0915 anti-mouse VISTA (PD-1H)


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