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APC/Cyanine7 anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136_APCCyanine7_NK11_Antibody_1_022324
C57BL/6 mouse splenocytes were stained with CD49b (clone DX5) FITC and NK1.1 (clone PK136) APC/Cyanine7 (left) or mouse IgG2a, κ APC/Cyanine7 isotype control (right).
  • PK136_APCCyanine7_NK11_Antibody_1_022324
    C57BL/6 mouse splenocytes were stained with CD49b (clone DX5) FITC and NK1.1 (clone PK136) APC/Cyanine7 (left) or mouse IgG2a, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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108723 25 µg $94
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108724 100 µg $292
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  2. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  3. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  4. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  5. Xu W et al. 2019. Immunity. 50(4):1054-1068 . PubMed
  6. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  7. Qiao Y, et al. 2021. Oncoimmunology. 10:1947569. PubMed
  8. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  9. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  10. Mitchell A, et al. 2012. J Immunol. 189:4970. PubMed
  11. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  12. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  13. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  14. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  15. Aldrich AL, et al. 2021. The Journal of Immunology. 206(4):751-765. PubMed
  16. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  17. Li J, et al. 2018. Immunity. 49:178. PubMed
  18. Brodin P, et al. 2010. PLoS One. 5:e13174. PubMed
  19. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  20. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  21. Goossens P, et al. 2019. Cell Metab. 29:1376. PubMed
  22. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  23. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  24. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  25. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  26. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  27. De Veirman K, et al. 2021. Oncoimmunology. 10:2000699. PubMed
  28. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  29. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  30. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  31. Etzerodt A, et al. 2019. J Exp Med. 216:2394. PubMed
  32. Lingegowda H, et al. 2022. Front Reprod Health. 3:726936. PubMed
  33. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  34. Barth ND, et al. 2018. J Immunol. 201:2602. PubMed
  35. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  36. Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed
  37. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
  38. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  39. Akoolo L, et al. 2021. Cell Microbiol. 23:e13350. PubMed
  40. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  41. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  42. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  43. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  44. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  45. Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed
  46. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  47. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  48. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  49. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  50. Etzerodt A, et al. 2020. J Exp Med. 217:00:00. PubMed
RRID
AB_830870 (BioLegend Cat. No. 108723)
AB_830871 (BioLegend Cat. No. 108724)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All NK-1.1 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse NK-1.1 PK136 FC
Biotin anti-mouse NK-1.1 PK136 FC
FITC anti-mouse NK-1.1 PK136 FC
PE anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
PE/Cyanine7 anti-mouse NK-1.1 PK136 FC
PE/Cyanine5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 488 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 647 anti-mouse NK-1.1 PK136 FC
Pacific Blue™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 711™ anti-mouse NK-1.1 PK136 FC
APC/Cyanine7 anti-mouse NK-1.1 PK136 FC
PerCP anti-mouse NK-1.1 PK136 FC
PerCP/Cyanine5.5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 700 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 421™ anti-mouse NK-1.1 PK136 FC,SB
Brilliant Violet 570™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 650™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 510™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 605™ anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 (Maxpar® Ready) PK136 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 785™ anti-mouse NK-1.1 PK136 FC
APC/Fire™ 750 anti-mouse NK-1.1 PK136 FC
TotalSeq™-A0118 anti-mouse NK-1.1 PK136 PG
Ultra-LEAF™ Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
TotalSeq™-B0118 anti-mouse NK-1.1 PK136 PG
TotalSeq™-C0118 anti-mouse NK-1.1 PK136 PG
PE/Fire™ 810 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 640 anti-mouse NK-1.1 PK136 FC
Spark UV™ 387 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 700 anti-mouse NK-1.1 PK136 FC
Go To Top Version: 2    Revision Date: 12/08/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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