LEGENDplex™ Buffer Set A2

Pricing & Availability
Regulatory Status
RUO
Other Names
Multiplex assay, flow cytometry, Buffer Set, COVID-19 Cytokine Storm Panel 1, COVID-19 Cytokine Storm Panel 2
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publications
Cat # Size Price  
741110 100 tests $100.00
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To purchase, view our LEGENDplex™ Mix and Match page

Description

The LEGENDplex™ Buffer Set A2 includes the following items:

  • Setup Beads
  • SA-PE
  • Assay buffer
  • Wash buffer
  • Plate Sealers
  • Matrix B2

LEGENDplex™ Buffer Set A2 is used in Mix and Match Subpanels of COVID-19 Cytokine Storm Panel 1 and Panel 2 with the following products:

741094 LEGENDplex™ COVID-19 Cytokine Storm Panel 1 and 2 Detection Antibodies
741095 LEGENDplex™ COVID-19 Cytokine Storm Panel 1 & 2 Standard
741096 LEGENDplex™ Human IL-6 Capture Bead A3, 14X
741097 LEGENDplex™ Human CCL2 (MCP-1) Bead A4, 14X
741098 LEGENDplex™ Human G-CSF Bead A5, 14X
741099 LEGENDplex™ Human IFN-α2 Bead A6, 14X
741100 LEGENDplex™ Human IL-2 Bead A8, 14X
741101 LEGENDplex™ Human IFN-γ Bead A10, 14X
741102 LEGENDplex™ Human IL-7 Bead B2, 14X
741103 LEGENDplex™ Human IL-1RA Bead B3, 14X
741104 LEGENDplex™ Human CXCL8 (IL-8) Bead B4, 14X
741105 LEGENDplex™ Human TNF-α Bead B5, 14X
741106 LEGENDplex™ Human CXCL10 (IP-10) Bead B6, 14X
741107 LEGENDplex™ Human CCL3 (MIP-1α) Bead B7, 14X
741108 LEGENDplex™ Human IL-10 Bead B9, 14X
741109 LEGENDplex™ Human CCL5 (RANTES) Bead A7, 14X
741113 LEGENDplex™ Human IL-13 Capture Bead A3, 14X
741114 LEGENDplex™ Human GM-CSF Bead A4, 14X
741115 LEGENDplex™ Human IL-1β Bead A5, 14X
741116 LEGENDplex™ Human IL-5 Bead A6, 14X
741117 LEGENDplex™ Human sCD25 (IL-2Ra) Bead A8, 14X
741118 LEGENDplex™ Human IL-4 Bead A10, 14X
741119 LEGENDplex™ Human VEGF Bead B2, 14X
741120 LEGENDplex™ Human IL-17A Bead B3, 14X
741121 LEGENDplex™ Human IL-18 Bead B4, 14X
741122 LEGENDplex™ Human APRIL Bead B5, 14X
741123 LEGENDplex™ Human CCL4 (MIP-1β) Bead B6, 14X
741124 LEGENDplex™ Human IL-15 Bead B7, 14X
741125 LEGENDplex™ Human IL-12p70 Bead B9, 14X
740377 LEGENDplex™ Filter Plate
OR
740379 LEGENDplex™ V-bottom Plate

Product Details
Technical data sheet

Product Details

Application

Multiplex


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.

Antigen Details

Gene ID
NA

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 1    Revision Date: 02/19/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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