Ultra-LEAF™ Purified anti-human CCL8 (MPC-2) Antibody

Pricing & Availability
Clone
A15150G (See other available formats)
Other Names
Monocyte chemotactic protein 2 (MCP-2), C-C Motif Chemokine Ligand 8
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A15150G_ULEAF_CCL8_Antibody_1_062320_updated.png
Recombinant human CCL8 (Cat. No. 581606) ( black circles) induces the chemotaxis of human THP-1 cells. Anti-human CCL8 antibody (clone W15150G) (purple squares) neutralizes the chemotaxis of recombinant human CCL8 at 0.2 ng/mL in human THP-1 cells in a dose-dependent manner while the Ultra-LEAF™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 400264) (grey triangles) does not have the effect. ND50 range: 0.4-4 µg/mL.
  • A15150G_ULEAF_CCL8_Antibody_1_062320_updated.png
    Recombinant human CCL8 (Cat. No. 581606) ( black circles) induces the chemotaxis of human THP-1 cells. Anti-human CCL8 antibody (clone W15150G) (purple squares) neutralizes the chemotaxis of recombinant human CCL8 at 0.2 ng/mL in human THP-1 cells in a dose-dependent manner while the Ultra-LEAF™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 400264) (grey triangles) does not have the effect. ND50 range: 0.4-4 µg/mL.
Cat # Size Price Quantity Avail. Save
933203 100 µg $245
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933204 1 mg $650
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Description

CCL8, also known as MCP-2, is a beta chemokine initially isolated from human MG-63 osteosarcoma cells stimulated with IL-1β. CCL8 (MCP-2) has structural and functional similarity with MCP-1 and MCP-3, and it is often co-induced with those chemokines in mononuclear cells. IFN-γ is a strong inducer of CCL8 in monocytes and fibroblasts. CCL8 can be processed and secreted as a truncated chemokine CCL8 (6-75). This isoform does not chemoattract monocytes, but it binds to the CCR2 receptor and induces its internalization; as a result, CCL8 inhibits MCP-1 (CCL2) and MCP-2 (CCL8) ERK signaling and antagonizes the chemotactic activity of several CCR2 ligands (MCP-1/CCL2, MCP-2/CCL8, MCP-3/CCL7). CCL8 is highly expressed in the skin, and it serves as an agonist for the chemokine receptor CCR8, but not for CCR2. Therefore, it has been speculated that the CCL8-CCR8 interaction is a crucial regulator of Th2 cell homing, which drives IL-5-mediated chronic allergic inflammation. In addition, CCL8 binds CCR5 receptor with high affinity and inhibits the replication of human immunodeficiency virus type 1. CCL8 is highly expressed in different human diseases, and it is a potential biomarker for the diagnosis of graft-versus-host diseases and tuberculosis.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human CCL8
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CCL8 (0.2 µg/mL) on human THP-1 cells. ND50 range: 0.4 - 4 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2861115 (BioLegend Cat. No. 933203)
AB_2861116 (BioLegend Cat. No. 933204)

Antigen Details

Structure
Chemokine
Distribution

Fibroblast, endothelial cells, monocytes, and macrophages

Function
CCL8 chemoattracts monocytes, T cells, NK cells, mast cells, eosinophils, and basophils. CCL8 is induced by IL-1β, IFNβ, INFγ, dsRNA, virus, ConA, and anti-CD2. The induction of CCL8 by IFNγ synergizes with the TLR ligands peptidoglycan (TLR2), dsRNA (TLR3), and LPS (TLR4).
Interaction
Monocytes, T cells, NK cells, mast cells, eosinophils, and basophils
Ligand/Receptor
CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195)
Cell Type
Basophils, Eosinophils, Mast cells, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Van Damme J, et al. 1992. J Exp Med. 176:59-65.
  2. Proost P, et al. 1998. J Immunol. 160:4034-41.
  3. Dean RA, et al. 2008. Blood. 112: 3455-64.
  4. Hori T, et al. 2008. Blood. 111:4403-12.
  5. Rom S, et al. 2010. FASEB J. 24:2292-300.
  6. Struyf S, et al. 2009. Eur J Immunol. 39:843-57.
  7. Islam SA, et al. 2011. Nat Immunol. 12:167-77.
Gene ID
6355 View all products for this Gene ID
UniProt
View information about CCL8 on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/09/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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