- Other Names
- Nephroblastoma overexpressed gene, C130088N23Rik
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NOV (CCN3) is a member of the CCN family of secreted, cysteine-rich regulatory proteins that share four conserved modules which confer distinct biological activities. These modules include an insulin-like growth factor binding domain, a von Willebrand type C domain, thrombospondin-1 domain, and a C-terminal cysteine knot domain. NOV (CCN3) is 354 amino acid (aa) protein that shares an 80% aa identity with mouse, rat, and canine NOV (CCN3). It is widely expressed developmentally, especially in the muscle, endothelium, nervous system, adrenal cortex, and chondrocytes. NOV (CCN3) acts through the Notch and BMP signaling pathways to modulate self-renewal and maturation of a number of cell lineages including hematopoietic, osteogenic, and chondrogenic lineages. NOV (CCN3) is also a putative ligand for integrin receptors and is tightly associated with the extracellular matrix. Its interaction with integrins alpha v beta 3 and alpha 5 beta 1 mediates endothelial cell adhesion induces chemotaxis and promotes angiogenesis. The elevated expression of NOV (CCN3) is associated with certain tumors such as Wilm’s tumor and most nephroblastomas.Product Details
- Mouse NOV, 344 amino acids (Ser26-Ile354) (Accession# Q64299) with C-terminal 10-His tag, was expressed in 293E cells.
- Molecular Mass
- The 344 amino acid recombinant protein has a predicted molecular mass of approximately 37.4 kD. The protein migrates at approximately 55 kD in DTT-reducing conditions and at approximately 54 kD in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Ser.
- >95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS, pH 6.5.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.) Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- ED50 = 0.2 - 0.8 µg/mL as measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org.
- Muscle, endothelium, nervous system, adrenal cortex, chrondrocytes, and hematopoietic progenitor cells.
- Endothelial cell chemotaxis, adhesion, and osteoclast, osteoblast, and myoblast differentiation.
- Endothelial cells, chondryocytes, osteoclasts, and osteoblasts.
- Integrins and Notch-1.
- NOV mediates Balb/3T3 mouse embryonic fibroblast cell adhesion.
- Biology Area
- Cell Adhesion, Angiogenesis
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
1. Snaith MR, et al. 1996. Genomics 38:425-428.
2. Bork P. 1993. FEBS Lett. 327:125-130.
3. Perbal B. 2006. Cell Commun. Signal 4:3.
4. Su BY, et al. 2001. Mol. Pathol. 3:184-91.
5. Gupta R, et al. 2007. Science 316:590-593.
6. Leu SJ, et al. 2002. J. Biol. Chem. 48:46248-55.
7. Lin CG, et al. 2003. J. Biol. Chem. 280:8229-37.
8. Wang X, et al. 2014. Biomaterials 9:2720-9.
9. Minamizato T, et al. 2007. Biochem. Biophys. Res. Commun. 2:567-73.
10. Wagener J, et al. 2013. Mol. Hum. Reprod. 4:237-49.
11. Sakamoto K, et al. 2002. J. Biol. Chem. 33:29399-405.
- Gene ID
- 18133 View all products for this Gene ID
- View information about NOV on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.