Recombinant Mouse IL-17A/F Heterodimer (carrier-free)

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Other Names
IL-17A/IL-17F
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IL-17A/F 072209
IL-6 induced by mIL-17A/IL-17F on fetal mouse skin fibroblasts.
  • IL-17A/F 072209
    IL-6 induced by mIL-17A/IL-17F on fetal mouse skin fibroblasts.
Cat # Size Price Quantity Avail. Save
580802 10 µg $195
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580804 25 µg $385
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580806 100 µg $1,000
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Description

IL-17A/F is part of the IL-17 cytokine family which consists of six structurally related proteins (IL-17A, B, C, D, E, and F). IL-17A is expressed primarily by Th17 cells, a subset of CD4 T cells. IL-17F is most closely related to IL-17A. The two molecules share 50% amino acid sequence homology. Like IL-17A, IL-17F mRNA and protein have been detected in Th17 cells. L-17F and IL-17A exist as a homodimers, adopting a cysteine knot motif formed through the interactions of four cysteines, one of which is responsible for the interchain bonding. IL-17F and IL-17A can form both homodimeric and heterodimeric proteins when expressed in a recombinant system and all forms of the recombinant proteins have in vitro functional activity. Mouse Th17 cells secret IL-17A/F as well as the homodimers IL-17A and IL-17F. IL-17A/F induces IL-6 and KC (CXCL1) production in mouse fibroblast and macrophages.IL-17A/F signals through IL-17A and TRAF6.

Product Details
Technical data sheet

Product Details

Source
Mouse IL-17A, amino acids Ala26-Ala158 (Accession # NM_010552) and mouse IL-17F, amino acids Arg29-Ala161 (Accession # NM_145856) were expressed independently in E. coli.
Molecular Mass
Recombinant mIL-17A/F is a disulfide linked heterodimer consisting of N-terminal methionylated mIL-17F and non-methionylated mIL-17A. The recombinant heterodimer has a predicted molecular mass of 29,974 Da. The DTT-reduced protein migrates as monomers, at approximately 15 and 16kDa by SDS-PAGE. The non-reduced protein migrates as a heterodimer, at approximately 30kDa by SDS-PAGE.
Purity
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 10 mM NaH2PO4, 0.15 M NaCl, pH 7.2.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The ED50 is 125 - 175 ng/ml, corresponding to a specific activity 0.57 - 0.8 x 104 units/mg, as determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of IL-6.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Cytokine
Distribution
IL-17A/F is expressed by Th17 cells and activated CD4 T cells
Interaction
IL-17A/F might act primarily on parenchymal cells such as fibroblasts, epithelial cells, and endothelial cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Chang SH, et al. Cell Res 17:435-440 2007.
2. Liang SC, et al. JI 179:7791-7799 2007.
3. Wright JF, et al. J. Biol. Chem. 282:13447-13455 2007.
4. Wright JF, et al. J. Immunol. 181:2799-2805 2008.
5. Fouser LA, et al. Immunol. Rev. 226:87-102 2008.

Gene ID
16171 View all products for this Gene ID 257630 View all products for this Gene ID
UniProt
View information about IL-17A/F on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 2    Revision Date: 08/19/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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