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Noggin (NOG) is an embryonic inducer that can sequester TGF-β cytokines of the BMP family with extremely high affinity. This protein was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. NOG also has been shown to modulate the activities of other BMPs including BMP-2, -7, -13, and -14. It is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle, and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of NOG to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons. NOG also appears to maintain adult stem cell populations in vivo. For example, it maintains neural stem cells within the hippocampus. Targeted deletion of NOG in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing NOG in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.Product Details
- Human Noggin, amino acids (Gln28-Cys232) (Accession# NP_005441.1), was expressed in HEK293 cells.
- Molecular Mass
- Recombinant human Noggin is a 46 kD disulfide-linked homodimer consisting of two 205 amino acid polypeptide chains. Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28 - 33 kD by SDS PAGE analysis under reducing conditions. The predicted N-terminal amino acid is Gln.
- >95%, as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Recombinant human Noggin is able to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC5 chondrogenic cells. The ED50 is 2.0 - 3.0 ng/ml, corresponding to a specific activity of 3.3 x 105 - 5.0 x 105 units/mg.
- Application Notes
This product is reactive with human, mouse, and chicken.
- Skeletal muscles, lungs, neurons, and skin
- Antagonizes BMPs
- Mesenchymal stem cells
- Human NOG is able to inhibit BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells.
- Cell Type
- Mesenchymal Stem Cells, Embryonic Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Signal Transduction, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
1. Valenzuela DM, et al. 1995. J. Neurosci. 15:6077.
2. McMahon JA, et al. 1998. Genes Dev. 12:1438.
3. Yuasa S, et al. 2005. Nat. Biotechnol. 23:607.
4. Gong Y, et al. 1999. Nat. Genet. 21:302.
5. Chiba S, et al. 2008. Stem Cells 26:2810.
6. Bonaguidi MA, et al. 2008. J. Neurosci. 28:9194.
- Gene ID
- 9241 View all products for this Gene ID
- View information about Noggin on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.