Recombinant Human LT-α (TNF-β)

Pricing & Availability
Other Names
Lymphotoxin-alpha (LT-α), Tumor necrosis factor-β, TNFSF1
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Cat # Size Price Quantity Avail. Save
562603 5 µg $125
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562604 20 µg $195
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Description

LT-α, also known as TNF-β, is a potent mediator of inflammatory and immune responses. It belongs to the TNF superfamily of ligands and signals through TNFR1 and TNFR2. LT-α is produced by activated T and B lymphocytes, and has similar activities to TNF-α. Like TNF-α, LT-α (TNF-β) is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. LT-α is secreted as a soluble polypeptide, but can form heterotrimers with LT-β, which effectively anchors the LT-α to the cell surface. LT-α is cytotoxic to a wide range of tumor cells. Recombinant human LT-α is secreted 171 amino acid protein (18.6 kD) which forms an active non-disulfide linked homotrimer structure in solution.

Product Details
Technical data sheet

Product Details

Source
E. coli, approximately 18.6 kD
Formulation
Sterile filtered through a 0.2 micron filter. Lyophilized from 0.3x PBS, pH 7.2.
Endotoxin Level
Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
The ED50 as determined by cytolysis of murine L929 cells in the presence of Actinomycin D is ≤0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Application

ELISA, Bioassay

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 2000 pg/ml to 16 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA: This LT-α protein is useful as a standard for a human LT-α sandwich ELISA, using unlabeled 359-238-8 antibody (catalog #503002) for capture and biotinylated 359-81-11 antibody (catalog #503104) for detection. Ligand Blocking: This LT-α protein is useful as a ligand-blocking specificity control for immunohistochemical or immunofluorescent staining. Bioassay: This LT-α protein is biologically active, and can be used for in vitro assays.

Antigen Details

Structure
TNF superfamily; trimer
Bioactivity
The ED50 as determined by cytolysis of murine L929 cells in the presence of Actinomycin D is ≤0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Cell Sources
Activated T and B cells, fibroblasts, astrocytes, myeloma, endothelial cells, epithelial cells
Cell Targets
Monocytes, B cells, fibroblasts, neutrophils, osteoclasts, keratinocytes, endothelial cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Aggarwal B, et al.Eds. 1992. Tumor necrosis factors:structure function and mechanism of action. Marcel Dekker Inc.
3. Bonavida B, et al.Eds. 1990. Tumor necrosis factor:structure mechanisms of action role in disease and therapy. Karger Basel.
4. Paul N, et al. 1987. Annu. Rev. Immunol. 6:407.

Regulation
Type II integral membrane protein, forms heterotrimer with type II integral membrane protein LT-β either as LTα1β2 or LTα2β1; processed secreted form is trimeric
Gene ID
4049 View all products for this Gene ID
UniProt
View information about LT-alpha on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 05/19/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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