Recombinant Human FGF-6 (carrier-free)

Pricing & Availability
Other Names
Fibroblast growth factor 6 (FGF-6), Heparin secretory-transforming protein 2 (HST-2), HSTF-2, Heparin-binding growth factor 6 (HBGF-6)
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Product Citations
publications
FGF-6_Human_RECOM_BA_092216
NIH3T3 proliferation induced by human FGF-6 In the presence of 1.5 µg/ml heparin.
  • FGF-6_Human_RECOM_BA_092216
    NIH3T3 proliferation induced by human FGF-6 In the presence of 1.5 µg/ml heparin.
Cat # Size Price Quantity Avail. Save
554202 10 µg $105
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554204 25 µg $190
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554206 100 µg $590
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554208 500 µg $1,640
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Description

FGF-6 is a member of the fibroblast growth factor (FGF) family, and it is closely related in structure to FGF-4. FGF-6 exhibits a very restricted expression profile predominantly in the developing muscle in embryos. FGF-6 mRNA can also be detected in testis, heart, and skeletal muscle in adults. FGF-6 is involved in both proliferation and differentiation of the myogenic lineages. In vitro studies have shown that the effects of FGF-6 are concentration dependent. At lower concentration, FGF-6 can increase the expression of many muscle cell differentiation markers. At higher concentrations, FGF-6 can downregulate the expression of myogenic markers and some myogenic transcription factors, and can delay C2 myogenic cell differentiation. Injection of FGF-6 recombinant protein into damaged mouse muscle can induce cyclin D1 mRNA and differentiation markers, including Cdkl1, MHC I and Tn1, which results in the acceleration of muscle regeneration. It has been shown that the expression of FGF-6 can transiently be upregulated by muscle injuries in both fast and slow myofibers. FGF-6 can stimulate migration of myogenic stem cells and also control the maintenance of muscle progenitor cells. During muscle regeneration, it has been shown that FGF-6 can be released from necrotic myofibers and be sequestered by basal laminae. FGF-6 regulates muscle differentiation through a calcineurin-dependent pathway and regulates myofiber size through IGF-II/TGF2R pathway. FGF-6 is also involved in bone metabolism.

Product Details
Technical data sheet

Product Details

Source
Human FGF-6, amino acids Gly41-Ile208 (Accession# P10767.4) with an N-terminal Met, was expressed in E. coli.
Molecular Mass
The 169 amino acid recombinant protein has a predicted molecular mass of approximately 18.9 kD. The DTT-reduced and non-reduced protein migrates at approximately 20 kD by SDS-PAGE. The predicted N-terminal amino acid is Met.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2, 10% glycerol and 1% CHAPS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The ED50 is 0.02 - 0.1 ng/mL, corresponding to a specific activity 1.0 - 5.0 x 107 units/mg, as determined by a dose-dependent proliferation of NIH3T3 cells in the presence of 1.5 µg/mL heparin.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Growth factor.
Distribution

Myogenic lineage cells, heart, and testis.

Function
FGF-6 plays important roles in myogenesis (proliferation, differentiation, and muscle regeneration) depending on concentration and alternative receptor usage. FGF-6 is upregulated by muscle injuries.
Interaction
Myogenic lineage cells, osteoblasts, and osteoclasts.
Ligand/Receptor
FGFR1 and FGFR4.
Cell Type
Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. DeLapeyriere O et al. 1993. Development. 118: 601.
2. Coulier, et al. 1994. Prog. Growth Factor Res. 5:1.
3. Pizette S, et al. 1996. Exp. Cell Res. 224:143.
4. Armand AS, et al. 2003. Biochim. Biophys. Acta. 1642:97.
5. Armand AS, et al. 2006. Biochim. Biophys. Acta. 1763:773.
6. Bosetti M, et al. 2010. J. Cell Physiol. 225:466.

Gene ID
2251 View all products for this Gene ID
UniProt
View information about FGF-6 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 09/22/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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