Recombinant Human CCL1 (I-309) (carrier-free)

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Other Names
T lymphocyte-secreted protein I-309
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CCL1_Human_RECOM_CF_BA_080816
BW5147 cells chemoattracted by human CCL1.
  • CCL1_Human_RECOM_CF_BA_080816
    BW5147 cells chemoattracted by human CCL1.
Cat # Size Price Quantity Avail. Save
582702 10 µg $125
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582704 25 µg $215
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582706 100 µg $585
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582708 500 µg $1,525
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Description

CCL1 was initially identified as a secreted protein derived from activated T cells. The CCL1/CCR8 axis is involved in the recruitment of Th2 effector cells in vivo to sites of allergic mucosal inflammation; as a result, there is an increase of CCR8+ CD4 T cell numbers in allergic asthma and a higher concentration of CCL1 in bronchoalveolar lavage of asthmatic patients compared with normal controls. In addition, high levels of CCL1 have been detected in the serum of patients with atopic dermatitis. CCL1 is constitutively expressed in normal skin, and CD8 and CD4 CCR8+ cells have been isolated from normal skin. Therefore, it has been suggested that CCL1/CCR8 play a role in skin immunosurveillance. In addition, a more recent publication showed that CCL1 transcript is upregulated in CD4 converted to Tregs by TGF-β in vitro, and this transcriptional upregulation is reversed by IL-6. This data suggests that CCL1 plays a role in Treg conversion. Also, antibodies against CCL1 inhibit the suppressive function of Tregs. Furthermore, the antitumor effect of CpG-ODN plus anti-CCL1 in BALB-neuT mice suggest that neutralization of CCL1 can be used as an adjuvant to antitumor immunotherapy.

Product Details
Technical data sheet

Product Details

Source
Human CCL1, amino acids Lys24-Lys96 (Accession# NM_002981.1) was expressed in E. coli.
Molecular Mass
The 74 amino acid recombinant protein has a predicted molecular mass of approximately 8.6 kD. The DTT-reduced protein migrates at approximately 13 kD, and the non-reduced protein migrates at approximately 15 kD by SDS-PAGE. The N-terminal amino acid is Lys.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to chemoattract mouse BW.5147.G.1.4OUAR.1 cells in a dose dependent manner. The ED50 = 0.5 - 2.5 ng/ml.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Chemokine
Distribution
T cells, Th1, Th2, diabetogenic T cells, peritoneal macrophages, peritoneal mesothelial cells, Langerhans cells, monocytes, mast cells, NK cells, endothelial cells, and epithelial cells
Function
CCL1 chemoattracts CD4 Th2 cells and plays a key role in the progression of Th2 type hypersensitivity
Interaction
T cells, Th2 cells, macrophages, microglia, FOXP3+ CD4 regulatory T cells, skin-homing CLA+T cells, monocytes, NK cells, dendritic cells, endothelial cells, and smooth muscle cells
Ligand/Receptor
CCR8 (CDw198)
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Miller MD, et al. 1989. J. Immunol. 143:2907.
2. Haque NS, et al. 2004. Blood 103:1296.
3. Gombert M, et al. 2005. J. Immunol. 174:5082.
4. Montes-Vizuet R, et al. 2006. Eur. Respir. J. 28:59.
5. Soler D, et al. 2006. J. Immunol. 177:6940.
6. Gonzalo JA, et al. 2007. J. Immunol. 179:1740.
7. Mutalithas K, et al. 2010. Clin. Exp. Allergy 40:1175.

Gene ID
6346 View all products for this Gene ID
UniProt
View information about CCL1 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 08/08/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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