Recombinant Human BDNF (ELISA Std.)

Pricing & Availability
Regulatory Status
RUO
Other Names
Brain-derived neurotrophic factor, ANON2, BLUN2
Ave. Rating
Submit a Review
Product Citations
publications
RECOM_HU_BDNF_ELISA_STD_121118.PNG
  • RECOM_HU_BDNF_ELISA_STD_121118.PNG
Cat # Size Price Quantity Check Availability Save
759709 4 pack $94
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Brain-derived neurotrophic factor (BDNF) is the second member of the NGF family of neurotrophic factors (neurotrophins) that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). BDNF shares approximately 55% amino acid identity with other family members. The amino acid sequence of mature BDNF is identical in all mammals examined. All neurotrophins are noncovalently linked homodimers and initially produced as proneurotrophins. These proneurotrophins have distinct biological activity different from their mature counterparts. Mature BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems during the development. BDNF participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

Product Details
Technical data sheet

Product Details

Source
Human BDNF, amino acids His129-Arg247 (Accession# P23560) was expressed in CHO cells.
Molecular Mass
The 119 amino acid recombinant protein has a predicted molecular mass of approximately 13.5 kD. The DTT-reduced and non-reduced proteins migrate at approximately 14.5 kD by SDS-PAGE. The predicted N-terminal amino acid is His.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 156.3 to 10,000 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This human BDNF protein is useful as a standard for a human BDNF sandwich ELISA, using unlabeled A15062G antibody (Cat. No. 536702) as capture and biotinylated A15062F antibody (Cat. No. 536804) as detection.

Antigen Details

Structure
Noncovalently linked homodimer
Distribution

Ubiquitous with high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung, and testis.

Function
Supports the survival of sensory and sympathetic neurons, and also dopaminergic midbrain neurons.
Interaction
Sensory neurons, sympathetic neurons, midbrain dopaminergic neurons.
Ligand/Receptor
TrkB
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Baloh RH, et al. 1998. Neuron. 21:1291
  2. Masure S, et al. 1999. Eur. J. Biochem. 266:892-902.
  3. Gardell LR, et al. 2003. Nat. Med. 9(11):1383-9.
  4. Honma Y, et al. 2002. Neuron. 35:267-82.
  5. Banerjee A, et al. 2012. PLoS One 7(11):e50098.
  6. Wong LE, et al. 2015. Proc. Natl. Acad. Sci. USA 112:6170-5.
  7. Zhang M, et al. 2016. Oncotarget. 7:3267-82.
Gene ID
627 View all products for this Gene ID
UniProt
View information about BDNF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/11/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login/Register
Remember me
Forgot your password? Reset Password
Request an Account