Purified anti-mouse CXCL12 (SDF-1β) Antibody

Pricing & Availability
Clone
M4201G11 (See other available formats)
Other Names
Pre-B-cell growth-stimulating factor (PBSF), stromal cell derived factor 1 isoform beta precursor (SDF-1ž), TLSF, TPAR1, SCYB12, IRH
Isotype
Rat IgG1, κ
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Product Citations
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532602 500 µg $495
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Description

SDF-1, also known as CXCL12, has several different splice variants, which include SDF-1α, SDF-1β, and SDF-1γ. SDF-1 is highly expressed in bone marrow stromal stem cells and regulates growth and survival. CXCL12 promotes survival by binding its receptor CXCR4 and inducing anti-apoptotic proteins, decreasing apoptotic proteins, and increasing transcription of cell survival genes. The SDF-1/CXCR4 pair plays a key role in maintaining proliferation and survival of bone marrow stem cell subpopulations under stress conditions. Data suggests that SDF-1β mediates cells' survival by enhancing autophagy in bone marrow mesenchymal stem cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
In-house recombinant mouse SDF1beta
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA - Quality tested
IP - Verified 

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of CXCL12 (SDF-1β) recombinant protein ranging from 2000 to 31.3 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

The purified M4201G11 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M4201G03 antibody as the detecting antibody.

For testing mouse CXCL12 (SDF-1ß) in serum, plasma, and cell culture supernatant, BioLegend's LEGEND MAX™ Kits are specially developed and recommended.This kit is specifically designed to accurately measure and distinguish SDF-1ß from SDF-1a.

RRID
AB_2686959 (BioLegend Cat. No. 532602)

Antigen Details

Structure
72 amino acids with a predicted molecular weight approximately at 8.5 kD.
Distribution

Expressed by bone marrow stromal cells, endothelial cells, epithelial cells, pericytes, dendritic cells, and fibroblasts.

Function
Apoptosis, innate immunity, renal development. SDF-1 is a constitutively produced chemokine involved in lung, brain, joint, and intestine inflammation. SDF-1 is chemotactic for T, B, and cancer cells. SDF-1/CXCR4 pair plays a role in the mobilization of CD34+ progenitors to peripheral blood. SDF-1 is important in morphogenesis, angiogenesis, and cancer metastasis.
Interaction
CXCR4 and ACKR3
Ligand/Receptor
CXCR4
Cell Type
Endothelial cells, Epithelial cells, Dendritic cells, Fibroblasts
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagasawa T, et al. 1994. Proc. Natl. Acad. Sci. USA 91:2305.
2. Muller A, et al. 2001. Nature 410:50.
3. Valenzuela-Fernandez A, et al. 2002. J. Biol. Chem. 277:15677.
4. De La Luz Sierra M, et al. 2004. Blood 103:2452.
5. Kortesidis A, et al. 2005. Blood 105:3793.
6. Davis DA, et al. 2005. Blood 105:4561.
7. Zlotnik A. 2008. J. Pathol. 215:211.
8. Herberg S, et al. 2013. PLoS One 8:e58207.

Gene ID
20315 View all products for this Gene ID
UniProt
View information about CXCL12 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03/27/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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