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Purified anti-human IL-33 Antibody

Pricing & Availability
Clone
Poly5163 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-33
Isotype
Goat Polyclonal Ig
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Product Citations
publications
Poly5165_Human_IL-33_103312
  • Poly5165_Human_IL-33_103312
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516301 100 µg $350
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Description

IL-33, also known as NFHEV and DVS 27, is a 30 kD proinflammatory protein that belongs to IL-1 superfamily. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1α or IL-1β stimulation. IL-33 interacts with IL1RL1/ST2 receptor and IL-1 receptor accessory protein (IL1RAP) in order to activate intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. Recombinant IL-33 has been shown to enhance Th2-associated immune responses and potently increase mast cell proliferation and cytokine production.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Human IL-33, amino acids Ser112-Thr270 (Accession# NM_ 033439) was expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by antigen affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 0.25-1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-33 recombinant protein ranging from 1000-15.6 pg/ml are recommended.

Application Notes

ELISA Capture: The purified Poly5163 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5163 antibody (Cat. No. 516303) as the detecting antibody and recombinant human IL-33 (Cat. No. 581809) as the standard.

RRID
AB_2561331 (BioLegend Cat. No. 516301)

Antigen Details

Structure
270 aa with predicted molecular weight of 30 kD.
Distribution
Full length IL-33 localizes to the nucleus in HUVECs and transfectants C-terminal fragment, corresponding mature IL-33 is secreted.
Function
Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T helper type 2-associated cytokines.
Interaction
Binding to IL1RL1/ST2 and IL-1 receptor accessory protein.
Cross
In sandwich ELISA, no cross-reactivity with mouse or rat IL-33.
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lohning M, et al. 1998. P. Natl. Acad. Sci. USA 95:693.
2. Onda H, et al. 1999. J. Cerebr. Blood F. Met. 19:1279.
3. Schmitz J, et al. 2005. Immunity 23:479.
4. McLaren JE, et al. 2010. J. Immunol. 185:1222.

Gene ID
90865 View all products for this Gene ID
UniProt
View information about IL-33 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All IL-33 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human IL-33 Poly5163 ELISA Capture
Biotin anti-human IL-33 Poly5163 ELISA Detection
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human IL-33

    Poly5165_Human_IL-33_103312

  • Biotin anti-human IL-33

    Poly5165_Human_IL-33_103312
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