- 14.1 (See other available formats)
- Other Names
- Mouse IgG2a
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- Product Citations
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Interleukin-16 is a proinflammatory cytokine synthesized as a 68 kD precursor molecule (pro-IL-16). After cleavage of pro-IL-16 by caspase-3, IL-16 is released as a 12 kD protein, but its biologic activities are exerted by a tetramer. IL-16 is a chemoattractant for CD4+ T cells, monocytes, and eosinophils. On T cells, IL-16 upregulates CD25 and class II expression, and also inhibits HIV-1 replication in vitro by repressing the transcription of the HIV-1 long terminal repeat. IL-16 is expressed by lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts. CD4 and CD9 are the receptors of IL-16.Product Details
- Human, Cross-Reactivity: Mouse
- Antibody Type
- Host Species
- Peptide corresponding to amino acids 615 to 630 (RRKSLQSKETTAAGDS) of IL-16
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ICFC - Quality tested
ELISA, IHC-F, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: ELISA2, inhibition of T cell chemotaxis2 and immunohistochemical staining of frozen lymph node sections3.
(PubMed link indicates BioLegend citation)
- Cruikshank WW, et al. 1994. P. Natl Acad Sci USA 91:5109. (ICFC)
- Keane J, et al. 1998. J. Immunol. 160:5945. (Block, ELISA)
- Chupp GL, et al. 1998. J. Immunol. 161:3114. (Block, IHC)
- Stoitzner P, et al. 2001. J. Invest. Dermatol. 116:641. (ICFC)
- Ren F, et al. 2005. J. Immunol. 174:2738. (ICFC)
- Product Citations
AB_11204433 (BioLegend Cat. No. 519102)
- 12 kD protein with a PDZ domain, product of the cleavage of pro-IL-16 by caspase-3, forms tetramers.
Lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts.
- Chemoattractant for CD4+ T cells, monocytes, and eosinophils. Upregulates CD25 and class II expression on T cells. Inhibits HIV-1 replication.
- Cell Type
- Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Macrophages, Mast cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Antigen References
1. Bellon M, et al. 2010. Blood 115:2441.
2. Rofani C, et al. 2009. Stem Cells Dev. 18:671.
3. Cruikshank W and Little F. 2008. Crit. Rev. Immunol. 28:467.
4. Gu Y, et al. 2008. J. Immunol. 181:4418.
5. McFadden C, et al. 2007. J. Immunol. 179:6439.
6. Qi JC, et al. 2006. Blood 107:135.
- Gene ID
- 3603 View all products for this Gene ID
- View information about IL-16 on UniProt.org
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