Purified anti-human IL-1 RA Antibody

Pricing & Availability
Clone
JK1RA-1 (See other available formats)
Other Names
Interleukin-1 receptor antagonist, IL-1 inhibitor, IL-1 receptor antagonist protein (IRAP), IL-1 inhibitory activity, ILS, MG50, IL-1RN
Isotype
Mouse IgG1
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
509901 50 µg $90
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509902 500 µg $295
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Description

Interleukin-1 receptor antagonist (IL-1RA) is composed of three isoforms, one secreted (sIL-1Rα/IL-1Rα1), two intracellular (IL-1Rα2/IL-1Rα3) and produced by monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma cells, keratinocytes, and chondrocytes. It is upregulated by IL-1β, IL-4, IL-6 and downregulated by glucocorticoids. The JK1RA-1 antibody reacts with human interleukin-1 receptor antagonist (IL-1RA).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-1 receptor antagonist
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide, 0.02% Proclin 950.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 4-6 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified JK1RA-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5095 (Cat. No. 509501) antibody as the detecting antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Xi X, et al. 2011. Invest Ophthalmol Vis Sci. 52:4859. PubMed.
Product Citations
  1. Xi X, et al. 2011. Invest Ophthalmol Vis Sci. 52:4859. PubMed
RRID
AB_315547 (BioLegend Cat. No. 509901)
AB_315548 (BioLegend Cat. No. 509902)

Antigen Details

Structure
Cytokine; three isoforms, one secreted (sIL-1Rα/IL-1Rα1), two intracellular (IL-1Rα2/IL-1Rα3); 17 kD (Mammalian)
Bioactivity
Storage forms that are released upon cell death to limit inflammation caused by cell debris; competitive inhibitor of IL-1 receptor binding; antagonizes IL-1 activities without having agonist effects; does not initiate intracellular signal transduction pr
Cell Sources
Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma cells, keratino
Cell Targets
B cells, T cells, monocytes
Receptors
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Carter D, et al. 1990. Nature 344:633.
2. Chirivi R, et al. 1993. Cancer Res. 53:5051.
3. Holtkamp G, et al. 1999. Eur. J. Immunol. 29:215.
4. Kim J, et al. 2003. Gene Ther. 10:1543.

Regulation
Upregulated by IL-1β, IL-4, IL-6; downregulated by glucocorticoids
Gene ID
3557 View all products for this Gene ID
UniProt
View information about IL-1RA on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/13/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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