Purified anti-human CD255 (TWEAK) Antibody

Pricing & Availability
CARL-2 (See other available formats)
Other Names
TNF-like weak inducer of apoptosis, Apo-3 ligand (Apo3L), Death receptor 3-L (DR3-L), TNFSF 12, CD255
Mouse IgM, κ
Ave. Rating
Submit a Review
Product Citations
Human TWEAK transfected cells stained with purified Carl-2, followed by biotinylated anti-mouse IgG and Sav-PE
  • Carl-2_Purified_072007
    Human TWEAK transfected cells stained with purified Carl-2, followed by biotinylated anti-mouse IgG and Sav-PE
  • HumanTWEAK_061604
Cat # Size Price Quantity Avail. Save
308402 100 µg $165
Check Availability

Need larger quantities of this item?
Request Bulk Quote

TNF-like weak inducer of apoptosis (TWEAK) is a type II transmembrane TNF superfamily member with strong homology to TNF in its extracellular portion, though less potent in inducing apoptosis. It has been clustered as CD255. TWEAK transcript is expressed broadly in many adult and fetal tissues; however, the staining of human peripheral blood mononuclear cells with TWEAK-specific antibodies shows a more restricted pattern. TWEAK is expressed as membrane-bound and secreted forms. Interaction of TWEAK with its receptors (DR3, Fn14) promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, and apoptosis in a number of human cell lines. The CARL-2 antibody can neutralize the bioactivity of natural or recombinant TWEAK.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
hTWEAK/2PK-3 transfectant cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

ELISA Capture - Quality tested
FC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
ELISA Capture: The purified CARL-2 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated CARL-1 antibody (Cat. No. 308304) as the detecting antibody.
Neutralization1: In a dose-dependent manner, CARL-1 is more effective than CARL-21. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308403).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakayama, M. 2000. J. Exp. Med. 192:1373.
AB_2206239 (BioLegend Cat. No. 308402)

Antigen Details

TNF superfamily; 17 kD (Mammalian)

Normal tissue and tumor cells at low levels

Promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, apoptosis
Weak apoptotic induction; mediate CD4+ T-cell killing of macrophages; induces IL-6, IL-8, ICAM-1 in astrocytes; HUVEC proliferation
Cell Sources
Transcripts widely expressed and abundant in most tissues; protein efficiently secreted
Cell Targets
Macrophages, astrocytes, endothelial cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Chicheportiche, Y., et al. 1997. J. Biol. Chem. 272:32401.
3. Kaplan, M., et al. 2000. J. Immunol. 164:2897.
4. Saas, P., et al. 2000. Glia 32:102.
5. Wiley, S., et al. 2001. Immunity 15:837.

Type II integral membrane protein readily cleaved from cell surface
Gene ID
8472 View all products for this Gene ID
View information about CD255 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Insert Note Here
Save Close Clear
Lab Timer
Forgot your password? Reset Password
Request an Account