PE/Dazzle™ 594 Streptavidin

Pricing & Availability
Other Names
Streptavidin-Phycoerythrin/Dazzle™ 594, SAv-PE/Dazzle™ 594
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Cat # Size Price Quantity Avail. Save
405247 25 µg $95
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405248 100 µg $255
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Description

Streptavidin binds to biotin with high affinity. Streptavidin - PE/Dazzle™ 594 is useful for detecting biotinylated antibodies. The excitation of PE/Dazzle™ 594 by the 488 nm laser light induces a light emission maximum of 610 nm.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The Streptavidin-PE/Dazzle™ 594 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of Streptavidin-PE/Dazzle™ 594 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE/ Dazzle™ 594 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤0.06 µg per million cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin-PE/Dazzle™ 594 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Dazzle™ 594 is 540 kD and Streptavidin alone is 52 kD.

Product Citations
  1. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  2. Delacher M, et al. 2020. Immunity. 52:295. PubMed

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Go To Top Version: 3    Revision Date: 06/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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