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PE anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
publications
A20
SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
  • A20
    SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
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110707 50 µg $80
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110708 200 µg $214
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Weiberg D, et al. 2018. PLoS One. 13:e0205247. PubMed
  2. Takihara Y, et al. 2022. Commun Biol. 5:776. PubMed
  3. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  4. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  5. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  6. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  7. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  8. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  9. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  10. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  11. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  12. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  13. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  14. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  15. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  16. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  17. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  18. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  19. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  20. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  21. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  22. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  23. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  24. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  25. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  26. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  27. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  28. Prasad M, et al. 2021. Front Immunol. 12:644483. PubMed
  29. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  30. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  31. Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed
  32. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  33. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  34. Tang Y, et al. 2015. Exp Hematol. 43: 554-564. PubMed
  35. Hu Z, et al. 2017. Sci Rep. 7:41558. PubMed
  36. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  37. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  38. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  39. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  40. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  41. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  42. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  43. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  44. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  45. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  46. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  47. Bei T, et al. 2021. Molecules. 26:. PubMed
  48. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  49. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  50. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  51. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  52. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  53. Li C, et al. 2016. Nat Commun. 7:12632. PubMed
  54. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  55. Ma K, et al. 2022. iScience. 25:104347. PubMed
  56. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  57. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  58. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  59. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  60. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  61. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  62. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  63. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  64. Li J, et al. 2011. Dev Comp Immunol. 35:872. PubMed
  65. Pfau S, et al. 2016. Genes Dev. 30: 1395 - 1408. PubMed
  66. Danzer H, et al. 2020. Elife. 9:00. PubMed
  67. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  68. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  69. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  70. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  71. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  72. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  73. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  74. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  75. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  76. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  77. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_313496 (BioLegend Cat. No. 110707)
AB_313497 (BioLegend Cat. No. 110708)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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