PE anti-mouse CD255 (TWEAK) Antibody

Pricing & Availability
Clone
MTW-1 (See other available formats)
Other Names
TNFSF12, TNF-related weak inducer of apoptosis, CD255
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
MTW-1_PE_090707
Mouse TWEAK transfected cells stained with MTW-1 PE
  • MTW-1_PE_090707
    Mouse TWEAK transfected cells stained with MTW-1 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
120005 50 µg $110
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Description

TWEAK is a 27 kD protein also known as tumor necrosis factor superfamily member 12 (TNFSF12), TNF-related weak inducer of apoptosis, and CD255. TWEAK is a type II transmembrane protein expressed on a variety of cell types. A soluble form of TWEAK is produced by proteolytic processing. TWEAK binds to the TWEAK receptor, FN14, to induce apoptosis, NF-κB translocation, and promote angiogenesis and endothelial cell proliferation. It has also been implicated in the induction of inflammatory cytokines.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of ligand binding2. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 120009 & 120010).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakayama M, et al. 2003. Biochem. Biophys. Res. Commun. 306:819.
  2. Mittal A, et al. 2010. J. Cell Biol. 188:833. (Block) PubMed
RRID
AB_2206211 (BioLegend Cat. No. 120005)

Antigen Details

Structure
TNF superfamily member, type II membrane protein and secreted (after proteolytic processing); forms homotrimer, 27 kD
Distribution

Widely expressed; induced on activated CD4+ cells

Function
Weak inducer of apoptosis in some cell types, activates NF-κB, promotes angiogenesis and endothelial cell proliferation, induces inflammatory cytokine production
Ligand/Receptor
TWEAKR/FN14, possible interaction with TNFSF12/APO3
Cell Type
T cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Chicheportiche Y, et al. 1997. J. Biol. Chem. 272:32401.
2. Lynch CN, et al. 1999. J. Biol. Chem. 274:8455.
3. Schneider P, et al. 1999. Eur. J. Immunol. 29:1785.

Gene ID
21944 View all products for this Gene ID
UniProt
View information about CD255 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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