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740762 LEGENDplex™ Human Fibrinolysis Panel Standard will be used together with the following Mix and Match Capture Beads and Human Fibrinolysis Panel Detection Antibodies, and Buffer Set D to offer flexible customization within the panel (5-plex):
740764 LEGENDplex™ Human Fibrinogen Capture Bead A4, 13X
740765 LEGENDplex™ Human Antithrombin Capture Bead A5, 13X
740766 LEGENDplex™ Human Plasminogen Capture Bead A6, 13X
740767 LEGENDplex™ Human Prothrombin Capture Bead B2, 13X
740768 LEGENDplex™ Human Factor XIII Capture Bead B5, 13X
740763 LEGENDplex™ Human Fibrinolysis Panel Detection Antibodies
740375 LEGENDplex™ Buffer Set D (Set Up Beads, Buffers, Plate Sealers, and SA-PE)
740377 or 740379 Filter Plate or V-bottom Plate
The LEGENDplex™ Human Fibrinolysis Panel is a multiplex bead-based assay panel, using fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 5 human proteins, including Fibrinogen, Antithrombin, Plasminogen, Prothrombin, and Factor XIII. This assay panel provides broader dynamic ranges than traditional ELISA methods. The panel has been validated for use with plasma samples.
- Gene ID
- If I don't have a vacuum, how do I remove the liquid from my plate?
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.