- Other Names
- Human Essential Immune Response Panel Standard
- Ave. Rating
- Submit a Review
- Product Citations
The LEGENDplex™ Human Essential Immune Response Panel Standard contains 13 human proteins, including IL-4, IL-2, CXCL10 (IP-10), IL-1β, TNF-α, CCL2 (MCP-1), IL-17A, IL-6, IL-10, IFN-γ, IL-12p70, CXCL8 (IL-8), Free Active TGF-β1. This standard cocktail is recommended for use with the following in Mix and Match assays:
Human Essential Immune Response Panel
740931 LEGENDplex™ Human Essential Immune Response Panel Detection Antibodies
740933 LEGENDplex™ Human IL-4 Capture Bead A4, 13X
740934 LEGENDplex™ Human IL-2 Capture Bead A5, 13X
740935 LEGENDplex™ Human CXCL10 (IP-10) Capture Bead A6, 13X
740936 LEGENDplex™ Human IL-1β Capture Bead A7, 13X
740937 LEGENDplex™ Human TNF-α Capture Bead A8, 13X
740938 LEGENDplex™ Human CCL2 (MCP-1) Capture Bead A10, 13X
740939 LEGENDplex™ Human IL-17A Capture Bead B2, 13X
740940 LEGENDplex™ Human IL-6 Capture Bead B3, 13X
740941 LEGENDplex™ Human IL-10 Capture Bead B4, 13X
740942 LEGENDplex™ Human IFN-γ Capture Bead B5, 13X
740943 LEGENDplex™ Human IL-12p70 Capture Bead B6, 13X
740904 LEGENDplex™ Human CXCL8 (IL-8) Capture Bead B7, 13X
740944 LEGENDplex™ Human Free Active TGF-β1 Capture Bead B9, 13X
740368 LEGENDplex™ Buffer Set A
740377 LEGENDplex™ Filter Plate
740379 LEGENDplex™ V-Bottom Plate
- Biology Area
- COVID-19, Immunology
- Molecular Family
- Gene ID
- View information about Essential Immune Response on UniProt.org
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.