|442807||1 Pre-coated Plate||$425|
Erythropoietin (EPO) is the most important hormone regulating erythropoiesis. It is a glycoprotein also known as hematopoietin. EPO is mainly produced by kidney and liver in response to hypoxia to induce red blood cell production. Rat EPO is a secreted protein of 166 amino acids with a predicted molecular mass of ~18.5 kD. However, it migrates on SDS-PAGE at ~35-50 kD due to heavy glycosylation. EPO is closely related to thrombopoietin (TPO) and highly conserved across species, with rat EPO being 95% and 79% identical to mouse and human, respectively.
EPO functions through binding to its receptor, EPO R, a homodimer, on erythoid precursors, and promotes their differentiation and maturation. As expected, serum levels of EPO are elevated in response to various conditions that result in tissue hypoxia (e.g., blood loss, high altitude, cardiac or pulmonary disease, and anemia). Increased EPO levels can also be due to increased production by cancer cells of the liver and kidney. Decreased levels of EPO are often seen with chronic renal failure and other chronic diseases or chemotherapy, which causes anemia. Rat endogenous EPO and recombinant EPO share the same amino acid sequence, with differences in the glycosylation profiles.
The LEGEND MAX™ Rat EPO ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a rat monoclonal anti-EPO antibody. The Detection Antibody is a biotinylated polyclonal goat anti-EPO antibody. This kit is specifically designed for the accurate quantitation of rat EPO from cell culture supernatant, serum, plasma (heparin), tissue homogenates and other biological fluids. This kit is analytically validated with ready-to-use reagents.
- Kit Contents
- Anti-rat EPO Pre-coated 96 well Strip Microplate
- Rat EPO Detection Antibody
- Rat EPO Standard
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Product Citations
- 1.0 pg/mL
- Standard Range
- 8.0 - 500 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µl to 1,000 µl
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Polypropylene vials
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- 24335 View all products for this Gene ID
- View information about Erythropoietin on UniProt.org
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?
We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.