- Other Names
- Free Active TGF-β1 Pre-coated ELISA Kit
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- Product Citations
|437707||1 Pre-coated Plate||$425|
Transforming growth factor beta 1 (TGF-β1) is a member of the transforming growth factor beta superfamily of cytokines. TGF-β1 precursor contains 390 amino acids with an N-terminal signal peptide of 29 amino acids required for secretion from a cell, a 249 amino acids pro-region (latency associated peptide or LAP), and a 112 amino acids C-terminal region that becomes the active TGF-β1 upon activation.
Both LAP and TGF-β1 exist as homodimers in circulation, but the disulfide linked homodimers of LAP and TGF-β1 remain non-covalently associated, forming the small latent TGF-β1 complex (SLC, 100 kD). The large latent TGF-β1 Complex (LLC, 235 - 260 kD) contains a third component, the latent TGF-β binding protein (LTBP), which is linked to LAP by a single disulfide bond. The LTBP does not confer latency, but for efficient secretion of the complex to extracellular sites. Free active TGF-β1 can be released (activated) by many factors including enzymes and low or high pH.
TGF-β1 is nearly 100% conserved across mammalian species. It has diverse biological functions in multiple cellular processes such as regulating proliferation and differentiation of various cell types. TGF-β1 is also an important immunoregulatory cytokine, which is involved in the maintenance of self-tolerance, Th17 differentiation, and T cell homeostasis, etc.
It is expected that normal serum, plasma, or other biological fluid contains low concentration of free active TGF-β1 and high concentration of Latent TGF-β1. It is the free active form TGF-β1 that binds TGF-β receptor and exerts biological functions. However, it has been difficult to quantify the free active TGF-β1 because of insufficient sensitivities of most assay products currently available on the market. It is necessary to measure both the free active form and total TGF-β1 in biological samples to understand the TGF-β1 functions.
- Kit Contents
- Anti-TGF-β1 Pre-coated 96-well Strip microplate
- TGF-β1 Detection Antibody
- TGF-β1 Standard
- Avidin-HRP D
- Assay Buffer C
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Mouse, Human, Rat, Porcine, NHP
- Additional Product Notes
View more applications data for this product in our Scientific Poster Library.
(PubMed link indicates BioLegend citation)
- Product Citations
- 2.3 pg/mL
- Standard Range
- 7.8-500 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Polypropylene vials
- Cell Sources
- Many cell types, highly expressed on activated Tregs and platelets
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- 21803 View all products for this Gene ID
- View information about TGF-beta1 on UniProt.org
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?
We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.