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KIRAVIA Blue 520™ anti-human IgG Fc Antibody

Pricing & Availability
Clone
M1310G05 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M1310G05_KB520_IgG_Fc_Antibody_042121.png
Human PBMCs were stained with anti-human IgG Fc (clone M1310G05) KIRAVIA Blue 520™ (left) or rat IgG2a, κ KIRAVIA Blue 520™ isotype control (right). Cells were costained with anti-human CD19 APC.
  • M1310G05_KB520_IgG_Fc_Antibody_042121.png
    Human PBMCs were stained with anti-human IgG Fc (clone M1310G05) KIRAVIA Blue 520™ (left) or rat IgG2a, κ KIRAVIA Blue 520™ isotype control (right). Cells were costained with anti-human CD19 APC.
Compare all formats See KIRAVIA Blue 520™ spectral data
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410733 25 tests $159
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410734 100 tests $393
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Description

IgG Fc is a homodimer that is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Siglec-E-IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm.


KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Sony and the Sony logo are registered trademarks of Sony Corporation.
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone M1310G05 recognizes IgG in the membrane of memory B cells, has a stronger affinity for IgG1 and IgG3 than for IgG2 and IgG4, and does not cross react with IgD, IgE, or IgM.

RRID
AB_2892474 (BioLegend Cat. No. 410733)
AB_2892474 (BioLegend Cat. No. 410734)

Antigen Details

Structure
Homodimer formed by the constant region of the IgG heavy chain.
Ligand/Receptor
CD16, CD32, and CD64.
Cell Type
B cells
Biology Area
Immunology
Antigen References

1. Paul, WE. (2003). Fundamental Immunology. Philadelphia, PA: Lippincott, Williams, & Wilkins.

Gene ID
3500 View all products for this Gene ID
UniProt
View information about IgG Fc on UniProt.org
Go To Top Version: 1    Revision Date: 04/22/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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