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Brilliant Violet 421™ anti-mouse/human IL-5 Antibody

Pricing & Availability
Clone
TRFK5 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)
Isotype
Rat IgG1, κ
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Product Citations
publications
TRFK5_BV421_IL-5_Antibody_FC_1_022014
PMA+ionomycin (6 hours)-stimulated Th2 polarized BALB/c splenocytes were stained with CD3 APC, and then intracellularly stained with IL-5 (clone TRFK5) Brilliant Violet 421™ or rat IgG1 Brilliant Violet 421™ isotype control (bottom).
  • TRFK5_BV421_IL-5_Antibody_FC_1_022014
    PMA+ionomycin (6 hours)-stimulated Th2 polarized BALB/c splenocytes were stained with CD3 APC, and then intracellularly stained with IL-5 (clone TRFK5) Brilliant Violet 421™ or rat IgG1 Brilliant Violet 421™ isotype control (bottom).
  • TRFK5_BV421_IL-5_Antibody_FC_2_022014
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
504311 50 µg $253
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Description

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Guinea Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially-purified T cell clone supernatant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

ELISA1,3,4 or ELISPOT2,6 Capture: The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Flow Cytometry: The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations.

Neutralization1,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318).

Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections7, and immunocytochemistry.

Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  2. Klinman, D., et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
  3. Swain, S., et al. 1990. J. Immunol. 145:3796.
  4. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  5. Assenmacher, M., et al. 1994. Eur. J. Immunol. 24:1097.
  6. Karulin, A., et al. 2000. J. Immunol. 164:1862.
  7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Fan, W. Y., et al. 2001. Exp. Biol. Med. 226:1045.
  9. Sitkauskiene, B., et al. 2005. Respir Res. 6:33.
Product Citations
  1. Fu Z, et al. 2019. Cell Rep. 28:159. PubMed
  2. Golebski K, et al. 2019. Nat Commun. 10:2162. PubMed
  3. Kaur K, et al. 2021. Cell Rep. 37:110178. PubMed
  4. Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed
  5. Chinthrajah S, et al. 2019. JCI Insight. 4:e131347. PubMed
  6. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  7. Holly Bachus et al. 2018. Immunity. 50(1):225-240 . PubMed
  8. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  9. Surace L, et al. 2021. Nat Immunol. 22:1367. PubMed
  10. Fu Z, et al. 2021. Nat Commun. 12:4462. PubMed
  11. Bosnjak B, et al. 2021. Front Immunol. 12:772240. PubMed
  12. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  13. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
  14. Kohn EM, et al. 2022. J Immunol. 208:1417. PubMed
  15. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  16. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  17. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  18. Lambert EE, et al. 2020. Vaccines (Basel). 8:. PubMed
RRID
AB_2563161 (BioLegend Cat. No. 504311)

Antigen Details

Structure
Cytokine; dimer; 40-50 kD (Mammalian)
Bioactivity
Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources
Mast cells, T cells, eosinophils
Cell Targets
Eosinophils, B cells
Receptors
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immunol. 35:593.

Gene ID
16191 View all products for this Gene ID 3567 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All IL-5 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human IL-5 TRFK5 ICFC
PE anti-mouse/human IL-5 TRFK5 ICFC
Purified anti-mouse/human IL-5 TRFK5 ELISA Capture,CyTOF®,IHC,WB
Purified anti-mouse/human IL-5 (Maxpar® Ready) TRFK5 ELISA Capture,CyTOF®
Brilliant Violet 421™ anti-mouse/human IL-5 TRFK5 ICFC
Ultra-LEAF™ Purified anti-mouse/human IL-5 TRFK5 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC,IHC,WB
Go To Top Version: 1    Revision Date: 02/21/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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