- KU32-52 (See other available formats)
- Other Names
- IL-32alpha,beta,gamma,delta, Interleukin 32 alpha,beta,gamma,delta, Natural Killer Transcript 4 (NK4), TNF-α-inducible factor (TAIFa,b,c,d)
- Mouse IgG1, κ
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- Product Citations
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Interleukin 32 (IL-32),previously known as a transcript (NK4), is produced by mitogen-activated lymphocytes, by IFNγ-activated epithelial cells or by IL-12 and IL-18-activated NK cells.Its expression is increased following activation of T-cells by mitogens or the activation of NK cells by IL-2.IL-32 activates NF-κ-B and p38 MAPK cytokine signal pathways. It has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues. IL-32 exists in at least four differentially spliced isoforms (α, β, γ and δ)with predicted molecular weight: ~26 kD.IL-32α is the shortest and most abundant of four potential splice variants of the pro-inflammatory cytokine IL-32.Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32β, suggesting an intracellular function.Product Details
- Antibody Type
- Host Species
- Recombinant human IL-32α
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.2-1 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-32 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application
- Application Notes
ELISA1 Detection: The biotinylated KU32-52 antibody is useful as a detection antibody in a sandwich ELISA assay, when used in conjunction with purified KU32-07 or KU32-56 antibody as the capture antibody for measuring human IL-32.
Additional reported applications (for the relevant formats) include: Western blotting, immunofluorescence, and immunoprecipitation.
Clone KU32-52 has been shown to bind to all isoforms of IL-32 and clone KU32-56 is shown to be reactive towards IL-32 a, ß, d, but not the ? isoform.1 Clone KU32-09 does not work for Western blotting.6
(PubMed link indicates BioLegend citation)
- Kim KH, et al. 2008. J. Immunol. Methods 333:38.
- Greene CM, et al. 2009. Am J. Respir Crit Care Med. PubMed
- Li W, et al. 2010. J. Immunol. 185:5056. PubMed
- Soyka MB, et al. 2012. Allergy. DOI:10.1111/j.1398-9995.2012.02820.x. (FC, IF) PubMed
- Hirata T, et al. 2012. Int Immunol. 24:705. PubMed.
- Seo Eun-hee, et al. 2008. J. Microbiol. Biotechnol. 9:1606-1612.
AB_2124017 (BioLegend Cat. No. 513503)
Activated lymphocytes and NK cells
- Cell Type
- T cells
- Biology Area
- Molecular Family
- Antigen References
1. Kim KH, et al. 2008. J. Immunol. Methods 333:38.
2. Conti P, et al. 2007. Autoimmun. Rev. 6:131.
3. Chen Q, et al. 2006. Vitam Horm. 74:207.
4. Kim SH, et al. 2005. Immunity 22:131.
5. Cagnard N, et al. 2005. Eur. Cytokine Netw. 16:289.
6. Banda NK, et al. 2003. J. Immunol. 170:2100.
- Gene ID
- 9235 View all products for this Gene ID
- View information about IL-32 alpha beta gamma delta on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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