Biotin anti-human IL-10 Antibody

Pricing & Availability
Clone
JES3-12G8 (See other available formats)
Other Names
Interleukin-10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)
Isotype
Rat IgG2a, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
501501 50 µg $85
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501502 500 µg $230
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Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-12G8 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-12G8 antibody can neutralize the bioactivity of natural or recombinant IL-10.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS - expressed, recombinant human IL-10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 500 to 2 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection1-4 or ELISPOT Detection5: The biotinylated JES3-12G8 antibody is useful as a detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Ultra-LEAF™ purified JES3-9D7 antibody (Cat. Nos. 501427 & 501428) or purified JES3-19F1 antibody (Cat. No. 506802/506810) as capture antibody and recombinant human IL-10 (Cat. No. 571009) as the standard.
Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, neutralization, and immunocytochemistry.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  2. Gotlieb, W., et al. 1992. Cytokine 4:385. (ELISA)
  3. Yssel, H., et al. 1992 J. Immunol. 149:2378. (ELISA)
  4. Burdin, N., et al. 1993. J. Exp. Med. 177:295. (ELISA)
  5. Klinman, D., et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.
  6. Tazi, A., et al. 1999. J. Immunol.163:3511
  7. Andersson, J., et al. 1994. Immunology 83:16. (IHC)
Product Citations
  1. Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed
RRID
AB_315177 (BioLegend Cat. No. 501501)
AB_315178 (BioLegend Cat. No. 501502)

Antigen Details

Structure
Acid-labile cytokine; dimer; 35-40 kD (Mammalian)
Bioactivity
Inhibit IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate TH2 cell response; mast cell proliferation in
Cell Sources
Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
Cell Targets
T cells, B cells, mast cells, macrophages
Receptors
IL-10R (CDw210)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. de Waal-Malefyt, R., et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard, M., et al. 1992. Immunol. Today. 13:198.
4. Quesniaux, V. 1992. Research Immunol. 143:385.

Regulation
Production inhibited by IL-4, IL-10
Gene ID
3586 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 11/10/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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