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APC anti-human CD192 (CCR2) Antibody

Pricing & Availability
Clone
K036C2 (See other available formats)
Regulatory Status
RUO
Other Names
CKR2, CCR2A, CCR2B, CKR2A, CKR2B, CMKBR2, MCP-1-R, CC-CKR-2
Isotype
Mouse IgG2a, κ
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Product Citations
publications
K036C2_APC_CD192_Antibody_FC_062413
Human peripheral blood monocytes were stained with CD192 (CCR2) (clone K036C2) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
  • K036C2_APC_CD192_Antibody_FC_062413
    Human peripheral blood monocytes were stained with CD192 (CCR2) (clone K036C2) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
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357207 25 tests $149
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357208 100 tests $326
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Description

CCR2 is a chemokine receptor that binds monocyte chemoattractant proteins (MCP-1, 2, 3 and 4). Two spliced variants were initially described for CCR2 (CCR2A and CCR2B). These variants differ in their terminal carboxyl tails. Monocyte adhesion to the arterial endothelium and subsequent migration into the intima are central events in the pathogenesis of atherosclerosis. CCR2 and MCP-1 have been associated to atherosclerotic plaques. MCP-1 is induced by modified-LDL in endothelial cells and may trigger firm adhesion of monocytes to vascular endothelium under flow conditions. Local overexpression of MCP-1 at vessel walls induces infiltration of macrophages and formation of atherosclerotic lesions. Obesity induces an inflammatory state that is implicated in many clinically important complications, including insulin resistance, diabetes, atherosclerosis, and non-alcoholic fatty liver disease. CCR2 influences the development of obesity and associated adipose tissue inflammation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CCR2 DNA immunogen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Epitope of clone K036C2 has been mapped to amino acids 11-29 and 21-39 of human CCR2.

Application References

(PubMed link indicates BioLegend citation)
  1. Tanaka T, et al. 2022. Monoclon Antib Immunodiagn Immunother. 41(5):285-289 (Epitope)
Product Citations
  1. Gabriely G, et al. 2021. iScience. 24:103347. PubMed
  2. Hoang A, et al. 2014. J Leukoc Biol. 96:481. PubMed
  3. Mandl M, et al. 2014. PLoS One. 9:112140. PubMed
  4. Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
  5. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  6. Gonzalo–Gil E, et al. 2019. Elife. 8:e44360. PubMed
  7. Berry MR et al. 2017. Cell. 170(5):860-874 . PubMed
  8. Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed
  9. Huang T, et al. 2020. Front Oncol. 10:19. PubMed
  10. Yang J, et al. 2018. Mediators Inflamm. 2018:9031452. PubMed
  11. Tian Z, et al. 2022. Cancer Cell Int. 22:362. PubMed
  12. Yao W, et al. 2017. EBioMedicine. 10.1016/j.ebiom.2017.07.014. PubMed
RRID
AB_2562238 (BioLegend Cat. No. 357207)
AB_2562239 (BioLegend Cat. No. 357208)

Antigen Details

Structure
Chemokine receptor, GPCR, seven transmembrane receptor
Distribution

Monocytes, macrophages, immature dendritic cells, memory T cells

Function
Induces monocyte adhesion to endothelial cells and chemotaxis
Ligand/Receptor
CCL2 (MCP-1), CCL7 (MCP-3), CCL8 (MCP-2), CCL13 (MCP-4), CCL16 (HCC4)
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Wong LM, et al. 1997. J. Biol. Chem. 272:1038.
2. Papadopoulou C, et al. 2008. Cytokine 43:181.
3. Barlic J, et al. 2007. J. Leukoc. Biol. 82:226.
4. Gu L, et al. 1998. Mol. Cell 2:275.
5. Volpe S, et al. 2012. PLoS One. 7:e37208.

Gene ID
729230 View all products for this Gene ID
UniProt
View information about CD192 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 4    Revision Date: 09/04/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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