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Alexa Fluor® 647 anti-human Granulysin Antibody

Pricing & Availability
Clone
DH2 (See other available formats)
Regulatory Status
RUO
Other Names
GNLY, 519, LAG2, NKG5, LAG-2, D2S69E, TLA519
Isotype
Mouse IgG1, κ
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Product Citations
publications
DH2_A647_1_051010
Human peripheral blood lymphocytes surface stained with CD56 PE then intracellularly stained with DH2 Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom)
  • DH2_A647_1_051010
    Human peripheral blood lymphocytes surface stained with CD56 PE then intracellularly stained with DH2 Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom)
  • DH2_A647_2_051010
Compare all formats See Alexa Fluor® 647 spectral data
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348006 100 tests $293
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Description

Granulysin is a member of the saposin-like protein family of lipid binding proteins (SAPLIP). It is expressed by NK cells and cytolytic T lymphocytes (CTL) as 9 kD and 15 kD forms. The 9 kD form is derived from the 15 kD form by cleavage at both amino- and carboxy-termini. Granulysin has broad cytolytic activities against a variety of tumor cells and microbes, including Gram-positive and -negative bacteria, fungi and parasites. It also functions as a chemoattractant for inflammatory cells and activates the cells expression of cytokines and chemokines.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant 9 kD granulysin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone DH2 recognizes both the 9 kD and 15 kD isoforms of Granulysin.1

ELISA Capture: To measure human granulysin, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated DH10 antibody (Cat. No. 526103) as the detection antibody. Recombinant Human Granulysin (ELISA Std.) (Cat. No. 552109) can be used as the protein standard.
Flow Cytometry: The fluorochrome-labeled DH2 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.
Note: For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 & 438008) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Hanson DA, et al. 1999. Mol. Immunol. 36:413.
  2. Spada FM, et al. 2000. J. Exp. Med. 191:937.
Product Citations
  1. Singh KS, et al. 2021. Nature. 589:597. PubMed
RRID
AB_2110110 (BioLegend Cat. No. 348006)

Antigen Details

Structure
Saposin-like protein family of lipid binding protein, 9 kD and 15 kD
Distribution

NK cells, cytolytic T cells

Function
Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells
Cell Type
NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Krensky AM. 2000. Biochem. Pharmacol. 59:317.
2. Deng A, et al. 2005. J. Immunol. 174:5243.
3. Kaspar AA, et al. 2001. J. Immunol. 167:350.
4. Krensky AM and Clayberger C. 2009. Tissue Antigens 73:193.

Gene ID
10578 View all products for this Gene ID
UniProt
View information about Granulysin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 11/24/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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