Biofunctional antibodies can mimic or interrupt the natural biological effects associated with ligand-receptor interaction. Or, they can directly affect a target cell or molecule. Because of these features, biofunctional antibodies are ideal tools for functional in vitro or in vivo assays. To meet your research or cell processing needs, explore our expanding line of GMP biofunctional antibodies - manufactured in a dedicated GMP facility and compliant with ISO 13485:2016 and MDSAP.
Our operating procedures include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016 and MDSAP
- QA review of released products
GMP Ultra-LEAF™ Purified anti-human CD3 SF Antibody
Our GMP Ultra-LEAF™ Purified anti-human CD3 SF Antibody (clone OKT3) is a potent activator of T cells, intended for use in the expansion of T cells for cell bioprocessing or other research applications. This biofunctional antibody is GMP manufactured under serum-free conditions, including serum-free hybridoma cell culture, without additional animal or human-derived materials. Each lot is tested for biofunctional activity measured by T cell expansion.
As our representative data show, our GMP serum-free OKT3 monoclonal antibody, in combination with anti-CD28, boasts similar bioactivity compared to our non-GMP product, thus ensuring it will be a potent tool to support your cell bioprocessing needs.
PBMC derived T cells were activated in the presence of Ultra-LEAF™ anti-human CD28 antibody (Cat. No. 302933) alone, or combined with Ultra-LEAF™ anti-human CD3 (Cat. No. 317326) or GMP Ultra-LEAF™ anti-human CD3 SF (Cat. No. 317354) for a period of 4 days. Activation was done via plate coating, with 1 μg/mL of each antibody. Cells were cultured for a total of 10 days, and viable cell number was determined.
GMP Ultra-LEAF™ Purified anti-GDNF Antibody
Glial cell-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily that signals through GDNF family receptor α-1 (GFRα1) to promote the development, differentiation, and survival of dopaminergic neurons. Because of its neuroprotective nature, GDNF is currently under investigation as a promising therapeutic for the treatment of Parkinson’s disease. We offer potent tools to study GDNF properties. Our GMP Ultra-LEAF™ Purified anti-GDNF antibody (purple squares, clone A16099G, Cat. No. 670905) inhibits the binding of recombinant human GFRα1 (black circles, Cat. No. 788102) to immobilized recombinant human GDNF (Cat. No. 760402) in a dose-dependent manner, while the isotype control (grey triangles, Cat. No. 401215) does not have the effect. This antibody neutralizes the binding of GFRα1 protein to GDNF, allowing researchers to examine neuronal growth and progression in disease and treatment models.