Recombinant Human IL-8 (ELISA Std.)

Pricing & Availability
Other Names
Interleukin-8, NAP-1, MDNCF, NAF, LAI, GCP, CXCL8
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Cat # Size Price Quantity Avail. Save
570909 4 pack 64€
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Technical Data Sheet (pdf)

Product Details

Source
Human IL-8, Ser 28- Ser 99 - Accession Number NM_000584, was expressed in E. coli.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration
Lot-specific (please contact technical support for mass/vial, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 1000pg/ml to 8pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IL-8 protein is useful as a standard for a human IL-8 sandwich ELISA, using unlabeled BH0814 antibody (catalog #514602) for capture and biotinylated BH0840 antibody (catalog #514704) for detection.

Antigen Details

Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
3576 View all products for this Gene ID
UniProt
View information about IL-8 on UniProt.org

Related FAQs

What is the murine homolog of IL-8?

Rodents lack a direct homologue of IL-8, but the chemokines CXCL1/KC, CXCL2/MIP-2, and CXCL5-6/LIX are regarded as functional homologues of IL-8.

Go To Top Version: 2    Revision Date: 02/29/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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