Purified anti-mouse CD47 Antibody

Pricing & Availability
Clone
miap301 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin-associated protein, IAP, neurophilin, gp42, Rh-associated protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
maip301_pure_CD47_Antibody_1_061708
C57BL/6 splenocytes stained with miap301 purified, followed by anti-rat IgG FITC
  • maip301_pure_CD47_Antibody_1_061708
    C57BL/6 splenocytes stained with miap301 purified, followed by anti-rat IgG FITC
  • maip301_pure_CD47_Antibody_2_053018
    C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mosue CD47 (clone miap301) and anti-mouse CD3e (clone 500A2) Alexa Fluor® 647 (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 Goat anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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127501 50 µg 40.00€
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127502 500 µg 92.00€
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Description

CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blockingand immunohistochemical staining of frozen sections3. This clone is not cross-reactive with human CD47.

Application References
  1. Wang H, et al. 2007. P. Natl. Acad. Sci. USA 104:13744.
  2. Jiang P, et al. 1999 J. Biol. Chem. 274:559. (IHC-F, Block)
Product Citations
  1. Podolnikova NP, et al. 2019. J Biol Chem. 294:7833. PubMed
RRID
AB_1089034 (BioLegend Cat. No. 127501)
AB_1089035 (BioLegend Cat. No. 127502)

Antigen Details

Structure
50 kD plasma membrane protein of the immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids, and a short cytoplasmic tail of 30 amino acids.
Distribution

Broad tissue distribution, including haemopoietic cells, epithelial and endothelial cells, fibroblasts, erythrocytes and other tissue cells.

Function
Adhesion molecules, immune regulation.
Interaction
Associates with β3 integrins.
Ligand/Receptor
SIRPα (CD172α, Signal regulatory protein alpha), thrombospondin.
Bioactivity
Upon interaction with SIRPα, controls innate immune effector function, such as host cell phagocytosis.
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Brown E, et al. 1990. J. Cell. Biol. 111:2785.
2. Mawby WJ, et al. 1994. Biochem. J. 304:525.
3. Gao AG, et al. 1996. J. Biol. Chem. 271:21.
4. Oldenborg PA, et al. 2000. Science 288:2051.
5. van Beek EM, et al. 2005. J. Immunol. 175:7781.

Gene ID
16423 View all products for this Gene ID
UniProt
View information about CD47 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 05/30/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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