PerCP anti-mouse CD45 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse thymus or spleen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation³, complement-dependent cytotoxicity^1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)^4,6, Western blotting⁷, and spatial biology (IBEX)^10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
8. Liu F, et al. 2012. Blood. 119:3295. PubMed
9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Filipello F et al. 2018. Immunity. 48(5):979-991 . PubMed
2. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
3. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
4. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
5. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
6. Quispe Calla N, et al. 2016. Sci Rep. 6:37723. PubMed
7. Bo Yu et al. 2018. Cell stem cell. 23(2):193-209 . PubMed
8. Keeter WC, et al. 2022. Eur Heart J Open. 2:oeac028. PubMed
9. Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed
10. Zhou J, et al. 2021. Int J Med Sci. 18:3516. PubMed
11. Sun J, et al. 2020. Acta Biomater. 289:106. PubMed
12. Shin J, et al. 2016. Sci Rep. 6:23426. PubMed
13. Kawashima N, et al. 2022. Nat Commun. 13:1624. PubMed
14. Lopez-Guadamillas E, et al. 2016. Sci Rep. 6:34542. PubMed
15. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
16. Stolp B, et al. 2022. Cell Rep. 38:110387. PubMed
17. Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed
18. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
19. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
20. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
21. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
22. Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
23. Tajfirouz D, et al. 2017. Virology. 10.1016/j.virol.2017.02.022. PubMed
24. Peng L, et al. 2020. Transl Lung Cancer Res. 0.892361111. PubMed
25. Liu YJ, et al. 2020. Theranostics. 10:5225. PubMed
26. Nguyen CM, et al. 2019. Diabetes. 68:1499. PubMed
27. Zhang C, et al. 2021. Clin Transl Immunology. 10:e1310. PubMed
28. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
29. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
30. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
31. Law HL, et al. 2020. Front Pharmacol. 11:274. PubMed
32. Sears SM, et al. 2020. Am J Physiol Renal Physiol. 319:F674. PubMed
33. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
34. Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed
35. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
36. Yang S, et al. 2019. Nat Commun. 10:2782. PubMed
37. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
38. Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed
39. Amo-Aparicio J, et al. 2021. Theranostics. 11:9805. PubMed
40. Saitoh BY, et al. 2021. Brain Behav Immun. 95:362. PubMed
41. Kumaran Satyanarayanan S, et al. 2019. Nat Commun. 10:3471. PubMed
42. Zou J, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:983. PubMed
43. Aebischer D, et al. 2014. PLoS One. 9:99297. PubMed
44. Everard A, et al. 2014. Nat Commun. 5:5648. PubMed
45. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
46. Demandt JAF, et al. 2021. Frontiers in Cell and Developmental Biology. 9:664258. PubMed
47. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
48. Wang F, et al. 2019. Cell Death Dis. 1.036111111. PubMed
49. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
50. Arcangeli S, et al. 2022. J Clin Invest. 132: . PubMed
51. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
52. Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed
53. Osaka M, et al. 2016. Sci Rep. 6:21391. PubMed
54. Davis M, et al. 2015. J Immunol. 194:2219. PubMed
55. West D, et al. 2014. J Immunol. 192:1762. PubMed
56. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
57. Oldrini B, et al. 2018. Nat Commun. 9:1466. PubMed
58. Xiong X et al. 2019. Mol Cell. 75(3):644-660 . PubMed
59. She L, et al. 2019. J Immunol. 203:2520. PubMed
60. Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
61. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
62. Wells KL, et al. 2020. Elife. 9:. PubMed
63. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
64. Rangaraju S, et al. 2018. Mol Neurodegener. 13:24. PubMed
65. Bogie JFJ, et al. 2021. J Autoimmun. 124:102723. PubMed
66. Kovacs M, et al. 2021. Acta Neuropathol Commun. 9:136. PubMed
67. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
68. Voss M, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L188 - L195. PubMed
69. Peñaloza HF, et al. 2018. Biochem Biophys Rep. 13:12. PubMed
70. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
71. Taddio MF, et al. 2021. Mol Imaging Biol. 23:196. PubMed
72. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
73. Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed
74. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
75. Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed
76. Li X, et al. 2021. Cell Death Dis. 12:314. PubMed
77. Woods S, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:912. PubMed
78. Enam S, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.07.013. PubMed
79. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
80. Doni A, et al. 2021. Nat Commun. 12:3739. PubMed
81. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
82. Gorina R, et al. 2014. J Immunol. 192:324. PubMed
83. Kerkhofs D, et al. 2020. Theranostics. 7.022222222. PubMed
84. Yamamuro T, et al. 2020. Nat Commun. 3.340277778. PubMed
85. Gomzikova MO, et al. 2020. Pharmaceutics. 12:00. PubMed
86. Yamasaki R, et al. 2014. J Exp Med. 211:1533. PubMed
87. Westrich JA, et al. 2019. Oncogene. 38:7166. PubMed
88. Liu Q, et al. 2021. Adv Mater. 33:e2102852. PubMed
89. Sears SM, et al. 2022. Kidney360. 3:818. PubMed
90. Wong L, et al. 2013. J Biol Chem. 288:35170. PubMed
91. Yin X, et al. 2015. J Immunol. 194:4029. PubMed
92. SL C, et al. 2016. JCI Insight. 1: 83116. PubMed
93. Cao Z, et al. 2021. Molecular Cell. 81(17):3604-3622.e10. PubMed
94. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
95. Bai C, et al. 2022. Exp Ther Med. 24:673. PubMed
96. Chen R, et al. 2019. Mol Med Rep. 1.172916667. PubMed
97. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
98. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
99. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
100. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
101. Chuang LT, et al. 2021. Molecules. 26:. PubMed

RRID

AB_893343 (BioLegend Cat. No. 103129)
AB_893339 (BioLegend Cat. No. 103130)

Antigen Details

Structure

Protein tyrosine phosphatase (PTP) family, 180-240 kD

Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function

Phosphatase, T and B cell activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1

Cell Type

B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells

Molecular Family

CD Molecules

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID

19264 View all products for this Gene ID

UniProt

View information about CD45 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

Certificate of Analysis